Detailed information of TA system TA220754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200710..201346
• Replicon: chromosome
• Accession: NZ_CP085415
• Organism: Priestia megaterium strain CK7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: LIT33_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 200996..201346 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: LIT33_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200710..200991 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT33_RS01105 (LIT33_01105)	196076..197047	+	972	WP_035439664.1	UV DNA damage repair endonuclease UvsE	-
LIT33_RS01110 (LIT33_01110)	197056..197658	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
LIT33_RS01115 (LIT33_01115)	197723..198088	+	366	WP_013055000.1	holo-ACP synthase	-
LIT33_RS01120 (LIT33_01120)	198147..199205	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
LIT33_RS01125 (LIT33_01125)	199319..200509	+	1191	WP_013055002.1	alanine racemase	-
LIT33_RS01130 (LIT33_01130)	200710..200991	+	282	WP_013055003.1	hypothetical protein	Antitoxin
LIT33_RS01135 (LIT33_01135)	200996..201346	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIT33_RS01140 (LIT33_01140)	201504..202340	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
LIT33_RS01145 (LIT33_01145)	202343..202699	+	357	WP_013055006.1	STAS domain-containing protein	-
LIT33_RS01150 (LIT33_01150)	202703..203104	+	402	WP_013055007.1	anti-sigma regulatory factor	-
LIT33_RS01155 (LIT33_01155)	203118..204128	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
LIT33_RS01160 (LIT33_01160)	204188..204520	+	333	WP_013055009.1	anti-sigma factor antagonist	-
LIT33_RS01165 (LIT33_01165)	204517..205002	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
LIT33_RS01170 (LIT33_01170)	204968..205762	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T220754 WP_013055004.1 NZ_CP085415:200996-201346 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T220754 NZ_CP117284:c2097422-2096655 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAAACTAGAAGAATTTAGTCAAGATAATTTTGAACAAGCCTCCAAACGACTCGTTCGTTCTTTAACTCGTGGAAAAAC
AACCTCATCTCAGCCTAAGGCTATTTTGCTCGGTGGACAGAGTGGTGCTGGGAAAACAACGATCCATCGAATTAAACAAA
GGGAATTTCAAGGTAATATCATTATTATTGATGGAGATAGTTATCGCTCTTTTCATCCGAACTACCTTGGCCTACAGGAA
AGGTATGGCAAAGATAGTGTGGATTATACCAAAGTATTCGCGGGACAAATGGTTGAATATCTTGTAGATGAGTTGAGTAA
AAAAGGTTATCATTTATTAATCGAGGGTACCTTGAGAACAACAGAAGTTCCCAAAAAAACTGCACAATTATTGACAACTA
AAGGTTACCAAGTCTCACTTGCAGTAATTGCGACCAAGCCAGAATTGTCCTATCTCAGTACCTTGATACGTTATGAAGAG
CTCCATGCTATAGACCCTAGTCAAGCTAGAGCGACTCCCAAAGAGCACCATGATGGAATTGTAGAACATTTAGTTGATAA
CTTACGAGAGTTAGAAAATGACAAAATTTTTAATCAAATTCAAATTTATCAAAGAGATCAATCGTGTATTTATGATTCGC
AAGTAGATCAAATCTCAGCAGCAGAAGTTTTACAAGAATGCCTCTTCGGAAAATGGAGTAAGGTGGAGGAAGAGATGTTG
AAGTTGGGACAGGAACGGTTGAAAGAGCTGGGGGAAATTGATAAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT220754 WP_013055003.1 NZ_CP085415:200710-200991 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT220754 NZ_CP117284:c2097898-2097422 [Streptococcus dysgalactiae subsp. equisimilis]
ATGATTGGAGAAAATATTAAAACGTTACGCAAGGCACATAATCTAACTCAGCCTGAGTTCGCAAAGATTGTTGGTATCTC
TCGAAATAGTCTGAGTCGTTATGAAAATGGGACAAGTCGAGTTTCGACAGAATTAATAGATCGTATCTGCAAGAAATTTA
ATGTTTCTTATATCGATATCGTAGGGGAGGAAAAAATGCTCACACCAGTAGAAGATTATCAGTTAACTCTGAAAATTGAA
GTGATTAAAGAACGAGGGGCGAATATCTTAGCGCAACTTTATCGTTTTCAAGATGAACAAGGAATTGCATTTGATGATAC
TTCTAATCCTTGGGTTCTAATGAGCGATGATTTGTCTGATTTAATTAACACGAAGATTTATCTTGTTGCAACTTTTGAGG
ATATAGAGCGATTTAATGGCTATTTGGATGGCATTGAACGTATGCTAGAACAAGCTACTCATCTGGTGGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9