Detailed information of TA system TA220751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2430686..2431661
• Replicon: chromosome
• Accession: NZ_CP085409
• Organism: Bacillus thuringiensis strain KF1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LIT28_RS12560
• Protein ID: WP_242253552.1
• Coordinates: 2430686..2431423 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LIT28_RS12565
• Protein ID: WP_061530834.1
• Coordinates: 2431536..2431661 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT28_RS12535 (LIT28_12535)	2426030..2426740	+	711	WP_242253221.1	class I SAM-dependent methyltransferase	-
LIT28_RS12540 (LIT28_12540)	2426861..2427268	+	408	WP_242253224.1	VOC family protein	-
LIT28_RS12545 (LIT28_12545)	2427316..2429001	-	1686	WP_242253226.1	alpha-keto acid decarboxylase family protein	-
LIT28_RS12550 (LIT28_12550)	2429109..2429591	+	483	WP_063223870.1	MarR family winged helix-turn-helix transcriptional regulator	-
LIT28_RS12555 (LIT28_12555)	2429760..2430497	+	738	WP_242253228.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
LIT28_RS12560 (LIT28_12560)	2430686..2431423	+	738	WP_242253552.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LIT28_RS12565 (LIT28_12565)	2431536..2431661	+	126	WP_061530834.1	hypothetical protein	Antitoxin
LIT28_RS12570 (LIT28_12570)	2431737..2431913	+	177	WP_242253230.1	stage II sporulation protein SB	-
LIT28_RS12575 (LIT28_12575)	2431957..2432232	-	276	WP_242253232.1	hypothetical protein	-
LIT28_RS12580 (LIT28_12580)	2432259..2432519	-	261	WP_252209273.1	hypothetical protein	-
LIT28_RS12585 (LIT28_12585)	2433178..2433354	+	177	WP_063223876.1	stage II sporulation protein SB	-
LIT28_RS12590 (LIT28_12590)	2433498..2434967	+	1470	WP_242253237.1	beta-Ala-His dipeptidase	-
LIT28_RS12595 (LIT28_12595)	2435563..2436033	-	471	WP_063223878.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.09 Da
• Isoelectric Point: 8.2998

>T220751 WP_242253552.1 NZ_CP085409:2430686-2431423 [Bacillus thuringiensis]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNSAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIMVPFEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T220751 NZ_CP117269:315768-315956 [Rhizobium rhododendri]
ATGAAGAGCGTCGATATCATTTCTGCTTTGACGAATGATGGTTGGTTTGAGGTTGCCAAGAAAGGCAGCCATGTCCAGCT
TAAACACCCGACAAAGCGAGGCCGTGTAACCGTTCCTCATCCTAAGCGAGATATCCCGATGGGGACGCTCAAGAGCATCG
AAAAGCAATCTGGGCTAAAACTGAGGTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4874.26 Da
• Isoelectric Point: 3.9652

>AT220751 WP_061530834.1 NZ_CP085409:2431536-2431661 [Bacillus thuringiensis]
VIKDNNNDFFKEDNEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT220751 NZ_CP117269:315962-316363 [Rhizobium rhododendri]
ATGCGCAACTATATCGGACTGATCCACAAGGAAGCTGAAAGCGATTACGGTGTTTCCTTTCCTGACTTCCCAGGTGTCGT
AACAGCCGGCACTGACTTGGATGACGCTCGTCGCATGGCCGAAGAGGCTTTGGCTTTTCACGTCGATGGTCTTGTCGAAG
ACGGAGAGGCTATCCCTGAAGCATCTTCCCTCACCGACATTATGGCCAATCCTCACAATTCTTCAGGTACCGCCATTCTT
GTTTCGCTAAAGACAGAAACAAAAAAGGCCGTCCGCGTAAACATCACGATCCCCGAGGATGTCTTGCAGGAGGTGGACGC
CTATGCAGAAGCACATGGTTTGACCAGGTCAGGATTTCTCGTTCAAGCAGCAAAGCGGGAGATCGGGAAGATTGCGGCTT
AG