Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242091..242733 Replicon chromosome
Accession NZ_CP085409
Organism Bacillus thuringiensis strain KF1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LIT28_RS01370 Protein ID WP_000635963.1
Coordinates 242383..242733 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LIT28_RS01365 Protein ID WP_000004570.1
Coordinates 242091..242378 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT28_RS01340 (LIT28_01340) 237406..238368 + 963 WP_242256766.1 UV DNA damage repair endonuclease UvsE -
LIT28_RS01345 (LIT28_01345) 238361..238933 - 573 WP_242256765.1 rhomboid family intramembrane serine protease -
LIT28_RS01350 (LIT28_01350) 239025..239384 + 360 WP_147808043.1 holo-ACP synthase -
LIT28_RS01355 (LIT28_01355) 239541..240491 + 951 WP_242256780.1 outer membrane lipoprotein carrier protein LolA -
LIT28_RS01360 (LIT28_01360) 240610..241779 + 1170 WP_242256764.1 alanine racemase -
LIT28_RS01365 (LIT28_01365) 242091..242378 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LIT28_RS01370 (LIT28_01370) 242383..242733 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIT28_RS01375 (LIT28_01375) 242802..244970 + 2169 WP_242256762.1 Tex family protein -
LIT28_RS01380 (LIT28_01380) 245028..245144 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LIT28_RS01385 (LIT28_01385) 245340..245798 + 459 WP_063224374.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T220750 WP_000635963.1 NZ_CP085409:242383-242733 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T220750 NZ_CP117268:c1171518-1170256 [Rhizobium rhododendri]
ATGACGGTAAGGGTCCTTAACGTCTGGTGGAATGGTCGCATTGTCGGACAGTTCACCCAAGATCAGCACGGCGACATTGG
CTTCGCCTACGCCGAAGCTTGGCTTGAGGACGAAAAGGCACTTCCGCTTTCTGTCTCCCTACCGAAGCGTCCAGAGCGTT
TCTCTCGCCGTGAATGCCGCCCATTTTTTGGCGGCCTACTGCCAGAGGAAAGCCAGCGTCTGGTTGCGGCGCAGGCGTTG
GGTGTTTCACCTGCCAACGACTTCGCCCTTCTTGATCGCTTGGGCGGCGACGTCGCCGGCGCGCTTCAACTTTTGCCCGA
AGACCAGGAACCGATTGGAGGGGGCCTGATTGCCGATCGGCAAACAGCAGCTCTCGATGAGGTCGGAATCGTCCGGATAC
TGGACGCACTTCCGACGAGGCCGCTCTTGGCCGGCCAGGAAGGCTTGAGGCTATCGCTCGCGGGCGCCCAATCAAAAGCT
CCAGTTGTTCTAATCGATGGCCAAGTCGCACTTCCCTTACCAGGCCAGGCGACGACGCACATCTTGAAGCCACCGATTGC
GCGCTTTCCAGGGACGACCGAGAATGAAGCCTTCGCGATGAGGCTTGCTGCCGCAATCGGGCTCGACGTTGCTCCCGTCG
ACCCGAGGTCCGCGAATGGTCGCCCTTTCCTTTTGGTCAAAAGATACGATCGCCACCACGATGGCGACGGTGTGGTTCTC
CGCATCCATCAGGAAGACTTCTGCCAAGCGCTGGGTGTGCCACCTGAAACAAAGTATGCAAGCGAGGGGGGGCCGACCTT
CAAGGATTGCTTCGAGCTGTTGCGTCGGGTCTCTGCGCGGCCGGGCACGGACATCGCTAAGCTTTTGGACGCTGCGATTT
TCAATCTCGTCATCGGAAATGCGGATGCTCACGGTAAGAATTTCTCGATCCTCTATGACCATTCTGGACCTAGGATGGCT
CCGCTGTACGACCTGCTTTCCACCGTGGCCTATCCAGATCTTTCCCCGAAATTGGCTATGCGGATCGGCAAACGCGCGAC
GCTTGCTGACATGGGCGCTGATGGCTGGCAGGCTTTCTCGAAGGAGACAGGTGTCAGCTTACCTTTAGTGCGCCGTCGGG
TGAAAGAACTGACTGATGCCACAGGGGACGCGATCCCCCGGTTATTGGAAGATATGTCCCCACTTGCCGCAGACCCTGTG
TCGTTAAATCGCGTAGCGGCTTTGATCGCAGACCGTGCCAAACTCGTGTCCCTGAGCATTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220750 WP_000004570.1 NZ_CP085409:242091-242378 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220750 NZ_CP117268:c1171745-1171515 [Rhizobium rhododendri]
ATGGCAAAACAAGTTAGTAGTCCAGCCGAGATCGGCGCCCTGGTCCGCAGCACACGCAAGGCGCAAAACTTGCGGCAGGA
CGAGCTTGCTGGCGTTTCTGGCGTCGGCCTGCGGTTTATCGTCGATCTCGAAGCCGGCAAACCGACAGCACAGATTGGAA
AGGTCTTGCAGGTTTTACAAACGCTCGGTTGTTCGATTGATATTCTAGCCCCAGGTGAACGTAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References