Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 262195..262837 Replicon chromosome
Accession NZ_CP085402
Organism Bacillus anthracis strain CMF9

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LIS83_RS01460 Protein ID WP_000635963.1
Coordinates 262487..262837 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LIS83_RS01455 Protein ID WP_000004570.1
Coordinates 262195..262482 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS83_RS01430 (LIS83_01430) 257511..258473 + 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -
LIS83_RS01435 (LIS83_01435) 258466..259038 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
LIS83_RS01440 (LIS83_01440) 259131..259490 + 360 WP_000635040.1 holo-ACP synthase -
LIS83_RS01445 (LIS83_01445) 259647..260597 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
LIS83_RS01450 (LIS83_01450) 260716..261885 + 1170 WP_000390600.1 alanine racemase -
LIS83_RS01455 (LIS83_01455) 262195..262482 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LIS83_RS01460 (LIS83_01460) 262487..262837 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIS83_RS01465 (LIS83_01465) 262905..265073 + 2169 WP_042513310.1 Tex family protein -
LIS83_RS01470 (LIS83_01470) 265131..265247 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LIS83_RS01475 (LIS83_01475) 265443..265901 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T220746 WP_000635963.1 NZ_CP085402:262487-262837 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T220746 NZ_CP117267:c1972300-1972007 [Rhizobium rhododendri]
ATGCTCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTCGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGGATGATTTGGAGATTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220746 WP_000004570.1 NZ_CP085402:262195-262482 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220746 NZ_CP117267:c1972006-1971704 [Rhizobium rhododendri]
TTGCCAGTGAAGACGCTACCTTTCGATGCTGCAGATCATTTGCAGAGCGGCCAATCCCAGCTTGAGTTGCTGAGCGATGC
TTTTAATTCCGGGAGCGCATCCTATGTCGCGGTGGCGTTGGGCGTCATTGCGCGCGCCAAAGGCATGACGGAAGTATCGA
AAACGGCAGGCATAACCCGCGAAGCGCTATACAAAGCTCTCAGCGCCGACGGCGATCCGAAGCTGAGCACGCTGCTTGGC
GTGCTCAACGCTCTCGACATCCAGCTGAAATTGGCCCCTCGCAACAAAAAAGCCCCGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References