Detailed information of TA system TA220745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2365909..2366884
• Replicon: chromosome
• Accession: NZ_CP085399
• Organism: Bacillus tropicus strain CK18

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LIS81_RS12165
• Protein ID: WP_061403070.1
• Coordinates: 2365909..2366646 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D8GY54
• Locus tag: LIS81_RS12170
• Protein ID: WP_000594608.1
• Coordinates: 2366759..2366884 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS81_RS12140 (LIS81_12145)	2360945..2361352	+	408	WP_226639077.1	VOC family protein	-
LIS81_RS12145 (LIS81_12150)	2361638..2362420	+	783	WP_226639086.1	class I SAM-dependent methyltransferase	-
LIS81_RS12150 (LIS81_12155)	2362542..2364227	-	1686	WP_252211340.1	alpha-keto acid decarboxylase family protein	-
LIS81_RS12155 (LIS81_12160)	2364334..2364816	+	483	WP_226639089.1	MarR family winged helix-turn-helix transcriptional regulator	-
LIS81_RS12160 (LIS81_12165)	2364983..2365720	+	738	WP_226639090.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
LIS81_RS12165 (LIS81_12170)	2365909..2366646	+	738	WP_061403070.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LIS81_RS12170 (LIS81_12175)	2366759..2366884	+	126	WP_000594608.1	hypothetical protein	Antitoxin
LIS81_RS12175 (LIS81_12180)	2366960..2367136	+	177	WP_226639091.1	stage II sporulation protein SB	-
LIS81_RS12180 (LIS81_12185)	2367160..2367549	-	390	WP_000808872.1	YxeA family protein	-
LIS81_RS12185 (LIS81_12190)	2367765..2369234	+	1470	WP_252211341.1	beta-Ala-His dipeptidase	-
LIS81_RS12190 (LIS81_12195)	2369815..2370285	-	471	WP_252211342.1	DUF5065 family protein	-
LIS81_RS12195 (LIS81_12200)	2370740..2371399	+	660	WP_252211343.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28405.13 Da
• Isoelectric Point: 8.2672

>T220745 WP_061403070.1 NZ_CP085399:2365909-2366646 [Bacillus tropicus]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T220745 NZ_CP117267:c1972006-1971704 [Rhizobium rhododendri]
TTGCCAGTGAAGACGCTACCTTTCGATGCTGCAGATCATTTGCAGAGCGGCCAATCCCAGCTTGAGTTGCTGAGCGATGC
TTTTAATTCCGGGAGCGCATCCTATGTCGCGGTGGCGTTGGGCGTCATTGCGCGCGCCAAAGGCATGACGGAAGTATCGA
AAACGGCAGGCATAACCCGCGAAGCGCTATACAAAGCTCTCAGCGCCGACGGCGATCCGAAGCTGAGCACGCTGCTTGGC
GTGCTCAACGCTCTCGACATCCAGCTGAAATTGGCCCCTCGCAACAAAAAAGCCCCGGACTGA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4878.27 Da
• Isoelectric Point: 3.9507

>AT220745 WP_000594608.1 NZ_CP085399:2366759-2366884 [Bacillus tropicus]
VIKNNDNDFFKEDNEDTTDFSLIKGDTMQQVEDLEDELKRK

Nucleotide

• Length: 126 bp

>AT220745 NZ_CP117267:c1972300-1972007 [Rhizobium rhododendri]
ATGCTCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTCGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGGATGATTTGGAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U6H9M7