Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 256185..256827 Replicon chromosome
Accession NZ_CP085399
Organism Bacillus tropicus strain CK18

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LIS81_RS01435 Protein ID WP_000635963.1
Coordinates 256477..256827 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LIS81_RS01430 Protein ID WP_000004570.1
Coordinates 256185..256472 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS81_RS01405 (LIS81_01405) 251501..252463 + 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -
LIS81_RS01410 (LIS81_01410) 252456..253028 - 573 WP_226641132.1 rhomboid family intramembrane serine protease -
LIS81_RS01415 (LIS81_01415) 253121..253480 + 360 WP_000635047.1 holo-ACP synthase -
LIS81_RS01420 (LIS81_01420) 253637..254587 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
LIS81_RS01425 (LIS81_01425) 254706..255875 + 1170 WP_000390607.1 alanine racemase -
LIS81_RS01430 (LIS81_01430) 256185..256472 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LIS81_RS01435 (LIS81_01435) 256477..256827 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIS81_RS01440 (LIS81_01440) 256895..259063 + 2169 WP_000426229.1 Tex family protein -
LIS81_RS01445 (LIS81_01445) 259121..259237 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LIS81_RS01450 (LIS81_01450) 259433..259891 + 459 WP_001975527.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T220744 WP_000635963.1 NZ_CP085399:256477-256827 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T220744 NZ_CP117267:1441741-1442043 [Rhizobium rhododendri]
ATGACCTCAGACACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAGCGAGAGTGCAATCGATGAATTTCTCGCTGCGGC
ATTCGAAACCGAGGACCCGGCATTTATCGCCAAAAGTCTTGGGGTGATTGCCAAAACCCGAAACATGAGCGCGATCGCCC
GAGATGTTGGAATGAGCCGTGCTGCCCTCTACAAATCGTTGAGCGGCAAAGGCAATCCGGAATTTGCGACCATTATAAAG
GTTATGAAGGCACTTGGCCTCGGCCTGGCTCCTGTCTCTCGGTCTAACCAGGGCGCTGCCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220744 WP_000004570.1 NZ_CP085399:256185-256472 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220744 NZ_CP117267:1441439-1441744 [Rhizobium rhododendri]
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTAGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGACGGTAACCTGCGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGTTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACGCAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCCGAATGGAAGGAGCGGAACCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References