Detailed information of TA system TA220743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 303717..304353
• Replicon: chromosome
• Accession: NZ_CP085398
• Organism: Rossellomorea marisflavi strain F17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LIT29_RS01640
• Protein ID: WP_048007230.1
• Coordinates: 304003..304353 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0J5SXN2
• Locus tag: LIT29_RS01635
• Protein ID: WP_048007231.1
• Coordinates: 303717..303998 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT29_RS01615 (LIT29_01610)	300011..300595	-	585	WP_048007234.1	rhomboid family intramembrane serine protease	-
LIT29_RS01620 (LIT29_01615)	300714..301064	+	351	WP_048007233.1	holo-ACP synthase	-
LIT29_RS01625 (LIT29_01620)	301262..302275	+	1014	WP_048007232.1	outer membrane lipoprotein carrier protein LolA	-
LIT29_RS01630 (LIT29_01625)	302402..303547	+	1146	WP_082139815.1	alanine racemase	-
LIT29_RS01635 (LIT29_01630)	303717..303998	+	282	WP_048007231.1	YlcI/YnfO family protein	Antitoxin
LIT29_RS01640 (LIT29_01635)	304003..304353	+	351	WP_048007230.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT29_RS01645 (LIT29_01640)	304749..305579	+	831	WP_048016254.1	RsbT co-antagonist protein RsbRA	-
LIT29_RS01650 (LIT29_01645)	305576..305932	+	357	WP_048007228.1	STAS domain-containing protein	-
LIT29_RS01655 (LIT29_01650)	305935..306336	+	402	WP_048007227.1	anti-sigma regulatory factor	-
LIT29_RS01660 (LIT29_01655)	306347..307357	+	1011	WP_048007226.1	PP2C family protein-serine/threonine phosphatase	-
LIT29_RS01665 (LIT29_01660)	307432..307764	+	333	WP_048007225.1	anti-sigma factor antagonist	-
LIT29_RS01670 (LIT29_01665)	307761..308231	+	471	WP_048007224.1	anti-sigma B factor RsbW	-
LIT29_RS01675 (LIT29_01670)	308209..309006	+	798	WP_252265644.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13007.02 Da
• Isoelectric Point: 5.1554

>T220743 WP_048007230.1 NZ_CP085398:304003-304353 [Rossellomorea marisflavi]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDSKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDDALQISVGLIQF

Nucleotide

• Length: 351 bp

>T220743 NZ_CP117267:1441439-1441744 [Rhizobium rhododendri]
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTAGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGACGGTAACCTGCGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGTTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACGCAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCCGAATGGAAGGAGCGGAACCAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10789.24 Da
• Isoelectric Point: 6.4767

>AT220743 WP_048007231.1 NZ_CP085398:303717-303998 [Rossellomorea marisflavi]
VSESSATTEILIRLPQQLVTELDGFADQENVNRNEFIYRATKMYLRERKKRHLRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT220743 NZ_CP117267:1441741-1442043 [Rhizobium rhododendri]
ATGACCTCAGACACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAGCGAGAGTGCAATCGATGAATTTCTCGCTGCGGC
ATTCGAAACCGAGGACCCGGCATTTATCGCCAAAAGTCTTGGGGTGATTGCCAAAACCCGAAACATGAGCGCGATCGCCC
GAGATGTTGGAATGAGCCGTGCTGCCCTCTACAAATCGTTGAGCGGCAAAGGCAATCCGGAATTTGCGACCATTATAAAG
GTTATGAAGGCACTTGGCCTCGGCCTGGCTCCTGTCTCTCGGTCTAACCAGGGCGCTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J5SXN2