Detailed information of TA system TA220741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 293009..293648
• Replicon: chromosome
• Accession: NZ_CP085395
• Organism: Peribacillus frigoritolerans strain CK6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LIT26_RS01510
• Protein ID: WP_034306610.1
• Coordinates: 293298..293648 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LIT26_RS01505
• Protein ID: WP_034306380.1
• Coordinates: 293009..293290 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT26_RS01485 (LIT26_01480)	289148..289738	-	591	WP_063595444.1	rhomboid family intramembrane serine protease	-
LIT26_RS01490 (LIT26_01485)	289846..290196	+	351	WP_034306384.1	holo-ACP synthase	-
LIT26_RS01495 (LIT26_01490)	290439..291443	+	1005	WP_053537120.1	outer membrane lipoprotein carrier protein LolA	-
LIT26_RS01500 (LIT26_01495)	291664..292848	+	1185	WP_286676012.1	alanine racemase	-
LIT26_RS01505 (LIT26_01500)	293009..293290	+	282	WP_034306380.1	antitoxin EndoAI	Antitoxin
LIT26_RS01510 (LIT26_01505)	293298..293648	+	351	WP_034306610.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT26_RS01515 (LIT26_01510)	294062..294889	+	828	WP_048682050.1	RsbT co-antagonist protein RsbRA	-
LIT26_RS01520 (LIT26_01515)	294892..295248	+	357	WP_048682053.1	STAS domain-containing protein	-
LIT26_RS01525 (LIT26_01520)	295252..295653	+	402	WP_048682056.1	anti-sigma regulatory factor	-
LIT26_RS01530 (LIT26_01525)	295663..296673	+	1011	WP_063595442.1	PP2C family protein-serine/threonine phosphatase	-
LIT26_RS01535 (LIT26_01530)	296733..297065	+	333	WP_034306369.1	anti-sigma factor antagonist	-
LIT26_RS01540 (LIT26_01535)	297062..297535	+	474	WP_034306366.1	anti-sigma B factor RsbW	-
LIT26_RS01545 (LIT26_01540)	297513..298301	+	789	WP_034306363.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12955.06 Da
• Isoelectric Point: 7.2029

>T220741 WP_034306610.1 NZ_CP085395:293298-293648 [Peribacillus frigoritolerans]
IVVKRGDVYFADLSPVVGSEQGGTRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEPMMQKVNEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T220741 NZ_CP117267:c929226-928930 [Rhizobium rhododendri]
ATGCCCTTGGAAACCACCCGCTTTGACATCCAGGACCATTTGACCACACCGGCCGACCGCATGCTCTATCTCGAAGCCGC
TTTCGAGGACGGTGATCCGGCGCTCATCACCCAGGCCCTCGGCGACGTTGCCCGTGCTGCCGGCATGACGTCTGTCGCCA
AAGAAGCGGGCGTGACCCGGGAAGCACTCTATAAATCACTGAGCGAAGCCGGTGATCCGCGCTTGTCGACCTTTCTCGGC
GTGATCAAGGCGCTAGGATTGAAGGTGTCGATCTCGACGGATCAGAATGCGGCTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10713.13 Da
• Isoelectric Point: 5.9054

>AT220741 WP_034306380.1 NZ_CP085395:293009-293290 [Peribacillus frigoritolerans]
VSESSATREILIRLPQNFLTELDGYASEENVNRSEFIYRATKMYLRERKKKEFRESMKRGYIEMAAINLTIASEAFQAEF
EAGHCVERLVSGG

Nucleotide

• Length: 282 bp

>AT220741 NZ_CP117267:c929517-929227 [Rhizobium rhododendri]
GTGATCGAAGTTCGGCAGACCTCTATTTTTGCGAATTGGCTAGATGCGCTCCGAGACGCGCAGGCGCGCCAACGCATCGT
CGTTCGCATCCGGCGGCTCGAACTCGGTAACCCTGGAGATATCAAGCCGGTCGGTGAAGGTGTCAGCGAAATGCGCATCC
CTCATGGCCCCGGCTACAGGCTCTATTTCACCCGATCCGGTGAGACGATCGTCATTCTTTTATGCGGCGGCGACAAGTCA
TCGCAGGCAAGGGACATCGCAATCGCCCGGCAATTGGCTAAGGAGATTTGA