220739

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 254461..255097 Replicon chromosome
Accession NZ_CP085394
Organism Bacillus sp. CMF12

Toxin (Protein)


Gene name mazF Uniprot ID W7KPR3
Locus tag LIT38_RS01380 Protein ID WP_009336311.1
Coordinates 254747..255097 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID W7L1R3
Locus tag LIT38_RS01375 Protein ID WP_035331931.1
Coordinates 254461..254742 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT38_RS01355 (LIT38_01360) 250507..251232 - 726 WP_243506591.1 rhomboid family intramembrane serine protease -
LIT38_RS01360 (LIT38_01365) 251397..251750 + 354 WP_243506592.1 holo-ACP synthase -
LIT38_RS01365 (LIT38_01370) 251895..252908 + 1014 WP_221881344.1 outer membrane lipoprotein carrier protein LolA -
LIT38_RS01370 (LIT38_01375) 253088..254245 + 1158 WP_252218074.1 alanine racemase -
LIT38_RS01375 (LIT38_01380) 254461..254742 + 282 WP_035331931.1 YlcI/YnfO family protein Antitoxin
LIT38_RS01380 (LIT38_01385) 254747..255097 + 351 WP_009336311.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIT38_RS01385 (LIT38_01390) 255477..256310 + 834 WP_243506596.1 RsbT co-antagonist protein RsbRA -
LIT38_RS01390 (LIT38_01395) 256307..256669 + 363 WP_009336309.1 STAS domain-containing protein -
LIT38_RS01395 (LIT38_01400) 256674..257075 + 402 WP_026580738.1 anti-sigma regulatory factor -
LIT38_RS01400 (LIT38_01405) 257087..258097 + 1011 WP_243506597.1 PP2C family protein-serine/threonine phosphatase -
LIT38_RS01405 (LIT38_01410) 258158..258490 + 333 WP_252216818.1 anti-sigma factor antagonist -
LIT38_RS01410 (LIT38_01415) 258487..258960 + 474 WP_009336304.1 anti-sigma B factor RsbW -
LIT38_RS01415 (LIT38_01420) 258938..259732 + 795 WP_243506598.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8998

>T220739 WP_009336311.1 NZ_CP085394:254747-255097 [Bacillus sp. CMF12]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Download         Length: 351 bp

>T220739 NZ_CP117267:500763-501035 [Rhizobium rhododendri]
ATGGCGTGGAGGATTGAGTTCCAGCGGTCAGCGGAACGCGATCTTGAAAAGCTCGACCATGAGAGCGTGAAAAGGATACT
CCGTTTCCTTCACACCCGTGTCGCCAGCCTCGAGGACCCCCGGTCGATAGGGGAGGCGCTTCGCGGATCGGAACTTGGAA
ACTTCTGGAAGTATCGTGTTGGAGACTATCGCATCATTGTCGACATAGACGACGGCACTGTCCGGATATTGGTCGTCCGC
ATTGGCAATCGGAAGGACGTCTATCGCCGCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10831.33 Da        Isoelectric Point: 7.2028

>AT220739 WP_035331931.1 NZ_CP085394:254461-254742 [Bacillus sp. CMF12]
VSESSTTTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT220739 NZ_CP117267:500555-500779 [Rhizobium rhododendri]
ATGCTTGCCCTGCGTTTGCCGCCTGAAATCGAAGCAAGACTAGATGAACTCGCCAAACGCACCGGGCGGAGCAAGAGCTT
CTATGCGCGTCAAGCGATATTGGAACACTTGGACGATCTGGAAGACCTCTACGTCGCCGAGCAAAGGCTCGAAGAATTCC
GTAGAGGCGACAGTGATAGTATTTCTCTCGCTGAGTTGATGGCGCGCCATGGCGTGGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A169FD46

Antitoxin

Source ID Structure
AlphaFold DB W7L1R3

References