220738

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type VII Classification (family/domain) HepT-MntA/HepT(toxin)
Location 61443..62244 Replicon plasmid unnamed1
Accession NZ_CP085393
Organism Cytobacillus oceanisediminis strain CK22

Toxin (Protein)


Gene name hepT Uniprot ID A0A160MHJ4
Locus tag LIT27_RS28390 Protein ID WP_019380940.1
Coordinates 61443..61847 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name mntA Uniprot ID A0A160MJA0
Locus tag LIT27_RS28395 Protein ID WP_019380939.1
Coordinates 61837..62244 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT27_RS28370 (LIT27_28425) 56807..57178 + 372 WP_019380943.1 DUF6527 family protein -
LIT27_RS28375 (LIT27_28430) 57471..58667 - 1197 Protein_59 IS110 family transposase -
LIT27_RS28380 (LIT27_28435) 59132..59725 + 594 WP_019380942.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
LIT27_RS28385 (LIT27_28440) 59758..60798 + 1041 WP_019380941.1 macro domain-containing protein -
LIT27_RS28390 (LIT27_28445) 61443..61847 - 405 WP_019380940.1 DUF86 domain-containing protein Toxin
LIT27_RS28395 (LIT27_28450) 61837..62244 - 408 WP_019380939.1 nucleotidyltransferase domain-containing protein Antitoxin
LIT27_RS28400 (LIT27_28455) 62736..63071 - 336 WP_019380938.1 (2Fe-2S) ferredoxin domain-containing protein -
LIT27_RS30260 (LIT27_28460) 63672..63911 - 240 WP_081757328.1 glutaredoxin domain-containing protein -
LIT27_RS28410 (LIT27_28465) 64285..64688 - 404 Protein_66 DUF86 domain-containing protein -
LIT27_RS28415 (LIT27_28470) 64678..65085 - 408 WP_019380934.1 nucleotidyltransferase domain-containing protein -
LIT27_RS28420 (LIT27_28475) 65278..65790 - 513 WP_019380933.1 DUF2269 family protein -
LIT27_RS28425 (LIT27_28480) 65787..66008 - 222 WP_019380932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..377383 377383


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease HepT-like

(14-131)

Antitoxin

Polymerase beta, nucleotidyltransferase

(7-98)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15480.04 Da        Isoelectric Point: 6.6378

>T220738 WP_019380940.1 NZ_CP085393:c61847-61443 [Cytobacillus oceanisediminis]
MKSDVILNKINIIERCIKRIHEEYDQNPKNLENYTKQDSIILNLQRACEASIDLAMHIVAVKKLGLPQQSRDAFTLLEAE
GIIPSSLSDKMKAMVGFRNIAVHDYQEINLVILQKILDNHLVDFFEYTKTILMH

Download         Length: 405 bp

>T220738 NZ_CP117267:162051-162380 [Rhizobium rhododendri]
GTGGATCGCGGAGACATCTGGCACGTCGATCTAGAACCGATCCGTGGACGTGAACAGGCTGGCGCGCGGTATGTGCTGGT
GGTTACCAAGAAAAGCTTCAATGCCATCGGCACGCCGATAGTGGTGCCTATAACGACCGGCAGTGAGTTTGCCCGTTTCA
AAGGATTTGCCGTGTCGTTGTCCGGCGCGGGAACGCGGAGTTCGGGTGTGATCCTGTGCAATCAGCTGCGGTCGCTCGAC
CTCAGGGCGCGCGGCGGGAAATACGTGGAAAGTGTGCCGGATTTCATCATGTTCGAAGTGCTTGCCCGGATCACTATATT
CTTCGACTAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 15464.67 Da        Isoelectric Point: 4.7024

>AT220738 WP_019380939.1 NZ_CP085393:c62244-61837 [Cytobacillus oceanisediminis]
MNPHLEETIIDVLREKLSPDWIVMFGSTVSGNTHKESDIDIAYLSDTIKADKYQIFMIAQELAAKLNKDVDLIDLNQAST
VFQAQIVQSGTTIYCTNERKKAEFEVKVLKMYTKLNEERSEILKKIDESGTIYEK

Download         Length: 408 bp

>AT220738 NZ_CP117267:161794-162051 [Rhizobium rhododendri]
ATGGCGACATCGACACTTCGCAATGTGGGAGGCTCTGTCATGATGACGATCCCGAAAGCTGTAGTTGATGAGTTGGGACT
GACAGCCAATACCAAGGTCGAGGTATTGGCAGAGGATGGGCGCGTCGTTGCGGTTCCGCACAAGCGGCCGAAGTACACGC
TCGATGAGCTTTTGGCGCAGTGCGATCTTGATGCACCGTTTACAGAGGAAGATCGGGAATGGCTCGATGATGCACCGTCT
GGCCGAGAGGTCATCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A160MHJ4

Antitoxin

Source ID Structure
AlphaFold DB A0A160MJA0

References