Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 255694..256330 Replicon chromosome
Accession NZ_CP085392
Organism Cytobacillus oceanisediminis strain CK22

Toxin (Protein)


Gene name mazF Uniprot ID W7KPR3
Locus tag LIT27_RS01380 Protein ID WP_009336311.1
Coordinates 255980..256330 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A160MHA2
Locus tag LIT27_RS01375 Protein ID WP_026041666.1
Coordinates 255694..255975 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT27_RS01355 (LIT27_01360) 251590..252315 - 726 WP_217027382.1 rhomboid family intramembrane serine protease -
LIT27_RS01360 (LIT27_01365) 252480..252833 + 354 WP_009336315.1 holo-ACP synthase -
LIT27_RS01365 (LIT27_01370) 252978..253991 + 1014 WP_019381248.1 outer membrane lipoprotein carrier protein LolA -
LIT27_RS01370 (LIT27_01375) 254160..255317 + 1158 WP_251267411.1 alanine racemase -
LIT27_RS01375 (LIT27_01380) 255694..255975 + 282 WP_026041666.1 CopG family ribbon-helix-helix protein Antitoxin
LIT27_RS01380 (LIT27_01385) 255980..256330 + 351 WP_009336311.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIT27_RS01385 (LIT27_01390) 256712..257545 + 834 WP_009336310.1 RsbT co-antagonist protein RsbRA -
LIT27_RS01390 (LIT27_01395) 257542..257904 + 363 WP_009336309.1 STAS domain-containing protein -
LIT27_RS01395 (LIT27_01400) 257909..258310 + 402 WP_009336308.1 anti-sigma regulatory factor -
LIT27_RS01400 (LIT27_01405) 258322..259332 + 1011 WP_019381250.1 PP2C family protein-serine/threonine phosphatase -
LIT27_RS01405 (LIT27_01410) 259393..259725 + 333 WP_009336305.1 anti-sigma factor antagonist -
LIT27_RS01410 (LIT27_01415) 259722..260195 + 474 WP_009336304.1 anti-sigma B factor RsbW -
LIT27_RS01415 (LIT27_01420) 260173..260967 + 795 WP_009336303.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8998

>T220737 WP_009336311.1 NZ_CP085392:255980-256330 [Cytobacillus oceanisediminis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Download         Length: 351 bp

>T220737 NZ_CP117262:c708093-706831 [Rhizobium tumorigenes]
ATGACGGTAAGGGTCCTTAACGTCTGGTGGAATGGTCGCATTGTCGGACAGTTCACCCAAGATCAGCACGGCGACATTGG
CTTCGCCTACGCCGAAGCTTGGCTTGATGACGAAAAGGCACTTCCGCTTTCTGTCTCCCTACCGAAGCGTCCAGAGCGTT
TCTCTCGCCGTGAATGCCGCCCATTTTTCGGCGGCCTACTGCCAGAGGAAAGCCAGCGTCTGGTTGCGGCGCAGGCGTTG
GGTGTTTCACCTGCCAACGACTTCGCGCTTCTCGATCGCTTGGGCGGCGACGTCGCCGGCGCGCTTCAACTTTTGCCCGA
AGACCAGGAACCGATTGTAGGGGGCCTGATTGCCGATCGGCAAACAGCAGCTCTCGATGAGGCCGGAATCGTCCGGATAC
TGGACGCACTTCCGACGAGGCCGCTCTTGGCCGGCCAGGAAGGCTTGAGGCTATCGCTCGCGGGCGCCCAATCAAAAGCT
CCAGTTGTTCTCATCGATGGCCAAGTCGCACTTCCCTTACCAGGCCAGGCGACGACGCACATCTTGAAGCCACCGATTGC
GCGCTTTCCAGGGACGACCGAGAATGAAGCCTTTGCGATGAGGCTTGCTGCCGCAATCGGGCTCGACGTTGCTCCCGTCG
ACCCGAGGTCCGCGAATGGTCGCCCTTTCCTTTTGGTCAACAGACACGATCGCCACCACGATGGCGACGGTGTGGTTCTC
CGCATCCATCAGGAAGACTTCTGCCAAGCGCTGGGTGTGCCACCTGAAACAAAGTATGCAAGCGAGGGGGGGCCGACCTT
CAAGGATTGCTTCGAGCTGTTGCGTCGGGTCTCTGCGCGGCCGGGCACGGACATCGCTAAGCTTTTGGACGCTGCGATTT
TCAATCTCGTCATCGGAAATGCGGATGCTCACGGTAAGAATTTCTCGATCCTCTATGACCATTCTGGACCTAGGATGGCT
CCGCTGTACGACCTGCTTTCCACCGTGGCCTATCCAGATCTTTCCCCGAAATTGGCTATGCGGATCGGCAAACGCGCGAC
GCTTGCTGACATGGACGCTGATGGCTGGCAGGCTTTCTCGAAGGAGACAGGTGTCAGCTTACCTTTAGTGCGCCGTCGGG
TGAAAGAACTGACGGATGCCACAGGGGACGCGATCCCCCGGTTATTGGAAGATATGTCCCCACTTGCCGCAGATCCTGTG
TCGTTAATTCGCGTAGCGGCTTTGATCGCAGACCGTGCCAAACTCGTGGCCCTGAGCATTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10817.30 Da        Isoelectric Point: 7.2025

>AT220737 WP_026041666.1 NZ_CP085392:255694-255975 [Cytobacillus oceanisediminis]
VSDSSTTTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT220737 NZ_CP117262:c708320-708090 [Rhizobium tumorigenes]
ATGGCAAAACAAGTTAGCAGTCCAGCCGAGATCGGCGCCCTGGTCCGCAGCACACGCAAGGCGCAAAACTTGCGGCAGGA
CGAGCTTGCTGGCGTTTCTGGCGTCGGCCTGCGGTTTATCGTCGATCTCGAAGCCGGCAAACCGACAGCACAGATCGGAA
AGGTCTTGCAGGTTTTACAAACGCTCGGTTGTTCGATCGATATTCTGGCCCCAGGTGAACGTAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A169FD46

Antitoxin

Source ID Structure
AlphaFold DB A0A160MHA2

References