Detailed information of TA system TA220736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2405541..2406465
• Replicon: chromosome
• Accession: NZ_CP085390
• Organism: Cytobacillus firmus strain CK19

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LIS77_RS12360
• Protein ID: WP_252222381.1
• Coordinates: 2405541..2406305 (+)
• Length: 255 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: LIS77_RS12365
• Protein ID: WP_222500259.1
• Coordinates: 2406286..2406465 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS77_RS12340 (LIS77_12345)	2400609..2402576	+	1968	WP_252222159.1	hypothetical protein	-
LIS77_RS12345 (LIS77_12350)	2402662..2403384	-	723	WP_252222162.1	hypothetical protein	-
LIS77_RS12350 (LIS77_12355)	2403660..2404883	+	1224	WP_252222164.1	S8 family peptidase	-
LIS77_RS12355 (LIS77_12360)	2404926..2405330	+	405	WP_222500264.1	hypothetical protein	-
LIS77_RS12360 (LIS77_12365)	2405541..2406305	+	765	WP_252222381.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LIS77_RS12365 (LIS77_12370)	2406286..2406465	+	180	WP_222500259.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LIS77_RS12370 (LIS77_12375)	2406729..2408099	-	1371	WP_252222167.1	aldehyde dehydrogenase	-
LIS77_RS12375 (LIS77_12380)	2408364..2409317	-	954	WP_222500255.1	nuclease-related domain-containing protein	-
LIS77_RS12380 (LIS77_12385)	2409373..2410626	-	1254	WP_252222175.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (4-239)

Antitoxin

• Antitoxin SpoIISB (16-58)

Sequences

Toxin

Protein

• Length: 255 a.a.
• Molecular weight: 29759.43 Da
• Isoelectric Point: 4.5024

>T220736 WP_252222381.1 NZ_CP085390:2405541-2406305 [Cytobacillus firmus]
MLVFFKVSVWMIVACLGFYVISVWLWEKKVKERMPVIRKTWYILYVLGAVVYWSTKPDSIFANWQNYLIVAVIFALVDAF
VFLGSYLKRVGNNELATDTRELLEENSDLLNSHMNKLKSFQYLLKNEPIDIYYGNKEAYLAGIEKLLADFAEKVDMKIAL
CKYTAKEEKDFLLEHYTDKALIHSRLDRREVYYDAEEKLALIPFSILEDDYVIKLTSDQFVTEFDYLLFTSLISIYDLML
PAEQEDEANEGTSE

Nucleotide

• Length: 765 bp

>T220736 NZ_CP117262:c155920-155501 [Rhizobium tumorigenes]
ATGATCTTGCTAGACACTAATGTAGTATCGGAGCCTTGGAAGCCGGCTCCTGATACTGCTGTGCTGACTTGGCTGGATGC
ACAGGCCATCGAGACGCTATTCATCTCTGCGATTTCAATTGCCGAACTTCGTTTCGGGATTGCCTCAATGCCCTCAGGAA
AGCGTCAGACAATTCTCCACGATCGCCTTGAAGGTGAAGTTCTTCCCATCTTTGACGGGCGCGTTCTGCCTTTTAACGTA
GGCACCGCGCAATTCTACGCGAAGCTGATGGCTAGCGCCCAAACATCAGGAAAGGCGATCGGGAAGGCTGATGGATACAT
TGCTGCTACGGCAGCAGAAAACCATCTCGCGGTCGCAACGCGAGATACAAGCCCATTTGAGGCAGCCGGAGTGAGAGTTA
TCAATCCTTGGGTCGCCTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6875.03 Da
• Isoelectric Point: 11.2836

>AT220736 WP_222500259.1 NZ_CP085390:2406286-2406465 [Cytobacillus firmus]
MKEPANKKKNVPRIHSNNAMKTLQKRSRISLPGFEVSPHTERILKENERLISVYTKTKT

Nucleotide

• Length: 180 bp

>AT220736 NZ_CP117262:c156171-155917 [Rhizobium tumorigenes]
GTGGCGGCTGTGACCATTCGAAACATCTCTGATGAAACGCATCGGGCATTACGCGCAAGAGCAACTCACCACGGCCGTAG
TACCGAGGCCGAAATTCGTGACATTCTTGAAGCCGCTGTTCGTCCATCTGGACGCGTGAAACTCGGATCATTGCTCGCGG
CCATTGGTCGTGATGCCAAACTGTCAGACAGTGACGTCGAAGCGCTGCAACAACAGCGCGACAGGACCCCGGCAGAACCG
ATGATTTTCGAATGA