Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 257341..257977 Replicon chromosome
Accession NZ_CP085390
Organism Cytobacillus firmus strain CK19

Toxin (Protein)


Gene name mazF Uniprot ID W7KPR3
Locus tag LIS77_RS01395 Protein ID WP_009336311.1
Coordinates 257627..257977 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID W7L1R3
Locus tag LIS77_RS01390 Protein ID WP_035331931.1
Coordinates 257341..257622 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS77_RS01370 (LIS77_01370) 252746..253471 - 726 WP_222501762.1 rhomboid family intramembrane serine protease -
LIS77_RS01375 (LIS77_01375) 253627..253980 + 354 WP_222501764.1 holo-ACP synthase -
LIS77_RS01380 (LIS77_01380) 254125..255138 + 1014 WP_197212719.1 outer membrane lipoprotein carrier protein LolA -
LIS77_RS01385 (LIS77_01385) 255720..256874 + 1155 WP_252220618.1 alanine racemase -
LIS77_RS01390 (LIS77_01390) 257341..257622 + 282 WP_035331931.1 YlcI/YnfO family protein Antitoxin
LIS77_RS01395 (LIS77_01395) 257627..257977 + 351 WP_009336311.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIS77_RS01400 (LIS77_01400) 258350..259183 + 834 WP_163144748.1 RsbT co-antagonist protein RsbRA -
LIS77_RS01405 (LIS77_01405) 259180..259542 + 363 WP_009336309.1 STAS domain-containing protein -
LIS77_RS01410 (LIS77_01410) 259547..259948 + 402 WP_048008772.1 anti-sigma regulatory factor -
LIS77_RS01415 (LIS77_01415) 259960..260970 + 1011 WP_252220620.1 PP2C family protein-serine/threonine phosphatase -
LIS77_RS01420 (LIS77_01420) 261031..261363 + 333 WP_035331808.1 anti-sigma factor antagonist -
LIS77_RS01425 (LIS77_01425) 261360..261833 + 474 WP_009336304.1 anti-sigma B factor RsbW -
LIS77_RS01430 (LIS77_01430) 261811..262605 + 795 WP_252220622.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8998

>T220735 WP_009336311.1 NZ_CP085390:257627-257977 [Cytobacillus firmus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Download         Length: 351 bp

>T220735 NZ_CP117261:2705779-2706069 [Rhizobium tumorigenes]
ATGGTTGAGAAATTAACCCCTTACGACCCAGCCGAAGATCTCGTCTCGGATGAGGCAATGGCAGTCTTCATGGGCGAAGC
GTTCGAGACGGGCGATGCCGGCTATATCGCCCACGCCCTTGGCGTGGTCGCGCGTGCAAAAGGCATGACGCAGATTGCCA
CCGACACCGGCCTGTCGCGGGAGCAACTCTACAGGTCTTTCAGTGAGGCGGGAAATCCAACCCTTAAGACAACGATGGCA
GTGATGAAGGCGCTCGGCATTACCCTCACCGCGAAGGTCGCAGCCGAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10831.33 Da        Isoelectric Point: 7.2028

>AT220735 WP_035331931.1 NZ_CP085390:257341-257622 [Cytobacillus firmus]
VSESSTTTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT220735 NZ_CP117261:2705481-2705786 [Rhizobium tumorigenes]
ATGTTGGAGCTGCGGCAAACAGCCGCTTTCGCAAAGTGGCGACAACGCCTGACCGATGACCGGGCCCGTGCCATAATCGC
CTCACGGCTCGACAGGCTTGCCTATGGCCACAGTGGCGACGCGCAGTCCGTCGGCGATGGCGTTCAGGAGCTTCGAATTC
AATATGGCCCCGGCTACCGCATATATTTTAAAACACGCGGCAACACGCTGATCATCCTGCTGTGCGGCAGCGACAAAAGC
ACGCAGGCAAGAGACATCAGCGCCGCAAAGCGCCTGCTGACGGAGTGGAGTGACGACCATGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A169FD46

Antitoxin

Source ID Structure
AlphaFold DB W7L1R3

References