Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 554547..555183 Replicon chromosome
Accession NZ_CP085389
Organism Bacillus altitudinis strain CMF5

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag LIS79_RS02715 Protein ID WP_003214169.1
Coordinates 554833..555183 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag LIS79_RS02710 Protein ID WP_003214273.1
Coordinates 554547..554828 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS79_RS02690 (LIS79_02690) 550701..551306 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
LIS79_RS02695 (LIS79_02695) 551401..551766 + 366 WP_017358398.1 holo-ACP synthase -
LIS79_RS02700 (LIS79_02700) 551927..552943 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
LIS79_RS02705 (LIS79_02705) 553072..554253 + 1182 WP_144741116.1 alanine racemase -
LIS79_RS02710 (LIS79_02710) 554547..554828 + 282 WP_003214273.1 hypothetical protein Antitoxin
LIS79_RS02715 (LIS79_02715) 554833..555183 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIS79_RS02720 (LIS79_02720) 555298..556128 + 831 WP_007496484.1 RsbT co-antagonist protein RsbRA -
LIS79_RS02725 (LIS79_02725) 556133..556501 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
LIS79_RS02730 (LIS79_02730) 556504..556905 + 402 WP_003214085.1 anti-sigma regulatory factor -
LIS79_RS02735 (LIS79_02735) 556916..557923 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
LIS79_RS02740 (LIS79_02740) 557983..558312 + 330 WP_007496474.1 anti-sigma factor antagonist -
LIS79_RS02745 (LIS79_02745) 558309..558797 + 489 WP_252224840.1 anti-sigma B factor RsbW -
LIS79_RS02750 (LIS79_02750) 558763..559551 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
LIS79_RS02755 (LIS79_02755) 559551..560150 + 600 WP_017358391.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T220734 WP_003214169.1 NZ_CP085389:554833-555183 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T220734 NZ_CP117261:2705481-2705786 [Rhizobium tumorigenes]
ATGTTGGAGCTGCGGCAAACAGCCGCTTTCGCAAAGTGGCGACAACGCCTGACCGATGACCGGGCCCGTGCCATAATCGC
CTCACGGCTCGACAGGCTTGCCTATGGCCACAGTGGCGACGCGCAGTCCGTCGGCGATGGCGTTCAGGAGCTTCGAATTC
AATATGGCCCCGGCTACCGCATATATTTTAAAACACGCGGCAACACGCTGATCATCCTGCTGTGCGGCAGCGACAAAAGC
ACGCAGGCAAGAGACATCAGCGCCGCAAAGCGCCTGCTGACGGAGTGGAGTGACGACCATGGTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT220734 WP_003214273.1 NZ_CP085389:554547-554828 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT220734 NZ_CP117261:2705779-2706069 [Rhizobium tumorigenes]
ATGGTTGAGAAATTAACCCCTTACGACCCAGCCGAAGATCTCGTCTCGGATGAGGCAATGGCAGTCTTCATGGGCGAAGC
GTTCGAGACGGGCGATGCCGGCTATATCGCCCACGCCCTTGGCGTGGTCGCGCGTGCAAAAGGCATGACGCAGATTGCCA
CCGACACCGGCCTGTCGCGGGAGCAACTCTACAGGTCTTTCAGTGAGGCGGGAAATCCAACCCTTAAGACAACGATGGCA
GTGATGAAGGCGCTCGGCATTACCCTCACCGCGAAGGTCGCAGCCGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References