Detailed information of TA system TA220733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1220044..1220961
• Replicon: chromosome
• Accession: NZ_CP085388
• Organism: Bacillus velezensis strain CMF18

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: LIS75_RS06420
• Protein ID: WP_003154806.1
• Coordinates: 1220215..1220961 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LIS75_RS06415
• Protein ID: WP_003154807.1
• Coordinates: 1220044..1220214 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS75_RS06375 (LIS75_06385)	1215275..1216897	+	1623	WP_252206759.1	pyocin knob domain-containing protein	-
LIS75_RS06380 (LIS75_06390)	1216910..1217281	+	372	WP_143255832.1	XkdW family protein	-
LIS75_RS06385 (LIS75_06395)	1217287..1217484	+	198	WP_044053582.1	XkdX family protein	-
LIS75_RS06390 (LIS75_06400)	1217541..1218302	+	762	WP_033575307.1	hypothetical protein	-
LIS75_RS06395 (LIS75_06405)	1218354..1218617	+	264	WP_003154815.1	hemolysin XhlA family protein	-
LIS75_RS06400 (LIS75_06410)	1218631..1218894	+	264	WP_003154813.1	phage holin	-
LIS75_RS06405 (LIS75_06415)	1218908..1219786	+	879	WP_014304858.1	N-acetylmuramoyl-L-alanine amidase	-
LIS75_RS06410 (LIS75_06420)	1219822..1219947	-	126	WP_003154809.1	hypothetical protein	-
LIS75_RS06415 (LIS75_06425)	1220044..1220214	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LIS75_RS06420 (LIS75_06430)	1220215..1220961	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LIS75_RS06425 (LIS75_06435)	1221066..1222064	-	999	WP_003154805.1	inorganic phosphate transporter	-
LIS75_RS06430 (LIS75_06440)	1222077..1222694	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LIS75_RS06435 (LIS75_06445)	1222980..1224296	-	1317	WP_003154801.1	amino acid permease	-
LIS75_RS06440 (LIS75_06450)	1224620..1225570	+	951	WP_060386897.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1186389..1228879	42490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T220733 WP_003154806.1 NZ_CP085388:c1220961-1220215 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T220733 NZ_CP117261:c2218580-2218365 [Rhizobium tumorigenes]
ATGGTATTGATCTTGTGGAGGCGCAGCGATCTTTGGATCGATCGCTTTTTGATTTCATTCCCCGCTCGGGACGGACTTGA
AAGTCGGTTTGTCGCGGTCGGTCAAATAGACGGGAAGCACTGGTCTTCGATTTGCACGAGACGCCATGAGAATATCAGGA
TCATTTCTGTTCGTCGGTCGAGGAAAGAGGAGATAGCTCTCTATGAAGGCAAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT220733 WP_003154807.1 NZ_CP085388:c1220214-1220044 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT220733 NZ_CP117261:c2218378-2218136 [Rhizobium tumorigenes]
ATGAAGGCAAGTGAACTCGACGAGAAATTCGACAATGGCGAGGATATACTCGACTATCTCGACCTCACAAAGGCAACCCG
CCCAAACATGGCGACCCAAAAGGTCAGCGTGGATTTTGCAACCTCAGTCGTAAATAGCCTTGACGAGGAAGCAGCCCGGC
TCGGGCTGACACGGCAATCACTGATCAATTTGTGGATTGAAGAGCGTCTGTCCGCCGCTCAGGATCAGAGAAAAGTATCT
TGA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14