Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 478880..479517 | Replicon | chromosome |
| Accession | NZ_CP085388 | ||
| Organism | Bacillus velezensis strain CMF18 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | LIS75_RS02485 | Protein ID | WP_003156187.1 |
| Coordinates | 479167..479517 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | LIS75_RS02480 | Protein ID | WP_003156188.1 |
| Coordinates | 478880..479161 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LIS75_RS02460 (LIS75_02470) | 475245..475844 | - | 600 | WP_015388676.1 | rhomboid family intramembrane serine protease | - |
| LIS75_RS02465 (LIS75_02475) | 475937..476302 | + | 366 | WP_003156192.1 | holo-ACP synthase | - |
| LIS75_RS02470 (LIS75_02480) | 476467..477474 | + | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| LIS75_RS02475 (LIS75_02485) | 477591..478760 | + | 1170 | WP_003156189.1 | alanine racemase | - |
| LIS75_RS02480 (LIS75_02490) | 478880..479161 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| LIS75_RS02485 (LIS75_02495) | 479167..479517 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| LIS75_RS02490 (LIS75_02500) | 479635..480456 | + | 822 | WP_014304404.1 | STAS domain-containing protein | - |
| LIS75_RS02495 (LIS75_02505) | 480461..480826 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| LIS75_RS02500 (LIS75_02510) | 480829..481230 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| LIS75_RS02505 (LIS75_02515) | 481242..482249 | + | 1008 | WP_025853725.1 | PP2C family protein-serine/threonine phosphatase | - |
| LIS75_RS02510 (LIS75_02520) | 482313..482642 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| LIS75_RS02515 (LIS75_02525) | 482639..483121 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| LIS75_RS02520 (LIS75_02530) | 483087..483875 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| LIS75_RS02525 (LIS75_02535) | 483875..484477 | + | 603 | WP_003156170.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T220732 WP_003156187.1 NZ_CP085388:479167-479517 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T220732 NZ_CP117261:1522688-1522990 [Rhizobium tumorigenes]
ATGACTTCAGATACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAACGAGAGTGCGGTCGACGAATTTCTCGCCGCGGC
GTTCGAAACCGAGGACCCGGCCTTCATCGCCAAAAGTCTTGGTGTGATTGCTAAAACCCGAAACATGAGCGCTCTCGCCC
GCGATGTCGGAATGAGCCGAGCTGCCCTCTACAAATCGCTGAGCGGCGAAGGCAATCCGGAATTTGCGACCATTATCAAG
GTTATGAAGGCACTTGGCCTCGGCCTTGCTCCAGTCTCGCGGTCCAACCAGGGCGCTGCCTGA
ATGACTTCAGATACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAACGAGAGTGCGGTCGACGAATTTCTCGCCGCGGC
GTTCGAAACCGAGGACCCGGCCTTCATCGCCAAAAGTCTTGGTGTGATTGCTAAAACCCGAAACATGAGCGCTCTCGCCC
GCGATGTCGGAATGAGCCGAGCTGCCCTCTACAAATCGCTGAGCGGCGAAGGCAATCCGGAATTTGCGACCATTATCAAG
GTTATGAAGGCACTTGGCCTCGGCCTTGCTCCAGTCTCGCGGTCCAACCAGGGCGCTGCCTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT220732 WP_003156188.1 NZ_CP085388:478880-479161 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT220732 NZ_CP117261:1522386-1522691 [Rhizobium tumorigenes]
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTCGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGATGGTAACCTGGGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGCTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACACAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCAGAATGGAGGGAGCGCAACCAATGA
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTCGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGATGGTAACCTGGGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGCTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACACAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCAGAATGGAGGGAGCGCAACCAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|