Detailed information of TA system TA220732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 478880..479517
• Replicon: chromosome
• Accession: NZ_CP085388
• Organism: Bacillus velezensis strain CMF18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LIS75_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 479167..479517 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LIS75_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 478880..479161 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS75_RS02460 (LIS75_02470)	475245..475844	-	600	WP_015388676.1	rhomboid family intramembrane serine protease	-
LIS75_RS02465 (LIS75_02475)	475937..476302	+	366	WP_003156192.1	holo-ACP synthase	-
LIS75_RS02470 (LIS75_02480)	476467..477474	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
LIS75_RS02475 (LIS75_02485)	477591..478760	+	1170	WP_003156189.1	alanine racemase	-
LIS75_RS02480 (LIS75_02490)	478880..479161	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LIS75_RS02485 (LIS75_02495)	479167..479517	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIS75_RS02490 (LIS75_02500)	479635..480456	+	822	WP_014304404.1	STAS domain-containing protein	-
LIS75_RS02495 (LIS75_02505)	480461..480826	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
LIS75_RS02500 (LIS75_02510)	480829..481230	+	402	WP_003156178.1	anti-sigma regulatory factor	-
LIS75_RS02505 (LIS75_02515)	481242..482249	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
LIS75_RS02510 (LIS75_02520)	482313..482642	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
LIS75_RS02515 (LIS75_02525)	482639..483121	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
LIS75_RS02520 (LIS75_02530)	483087..483875	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
LIS75_RS02525 (LIS75_02535)	483875..484477	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T220732 WP_003156187.1 NZ_CP085388:479167-479517 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T220732 NZ_CP117261:1522688-1522990 [Rhizobium tumorigenes]
ATGACTTCAGATACCAGACTTTTCGACGCTTCCGAGGTTCTCGACAACGAGAGTGCGGTCGACGAATTTCTCGCCGCGGC
GTTCGAAACCGAGGACCCGGCCTTCATCGCCAAAAGTCTTGGTGTGATTGCTAAAACCCGAAACATGAGCGCTCTCGCCC
GCGATGTCGGAATGAGCCGAGCTGCCCTCTACAAATCGCTGAGCGGCGAAGGCAATCCGGAATTTGCGACCATTATCAAG
GTTATGAAGGCACTTGGCCTCGGCCTTGCTCCAGTCTCGCGGTCCAACCAGGGCGCTGCCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT220732 WP_003156188.1 NZ_CP085388:478880-479161 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT220732 NZ_CP117261:1522386-1522691 [Rhizobium tumorigenes]
GTGTTCGTTATCCAGCGCACCGAAATCTTCCAGCAGTGGATTATGCGACTGAAGGATCGTCGCGCCGCCGCTCGCATTGC
ATCGCGCATTTTGCGCGCGGAAGATGGTAACCTGGGTGACGTCAAACCTGTCGGAGATGGCGTCGAGGAAATGCGTATCG
ACTACGGGCCGGGCTACAGGATCTATTTCCTCAGACGGCGCTCGGTGCTTGTCGTTCTTCTCGTCGGCGGCGACAAGAGC
ACACAGCGAAGAGACATCATCGAAGCAAAGCGCCTTTGTGCAGAATGGAGGGAGCGCAACCAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5