Detailed information of TA system TA220725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1950195..1951876
• Replicon: chromosome
• Accession: NZ_CP085344
• Organism: Cupriavidus sp. MP-37

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LIN44_RS09145
• Protein ID: WP_227311888.1
• Coordinates: 1950195..1951541 (-)
• Length: 449 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LIN44_RS09150
• Protein ID: WP_227311889.1
• Coordinates: 1951544..1951876 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN44_RS09115 (LIN44_09115)	1945762..1946883	+	1122	WP_227311885.1	hypothetical protein	-
LIN44_RS09120 (LIN44_09120)	1947557..1948474	+	918	WP_227311886.1	transcriptional regulator	-
LIN44_RS09125 (LIN44_09125)	1948683..1949765	+	1083	WP_227311887.1	alpha/beta hydrolase	-
LIN44_RS09145 (LIN44_09145)	1950195..1951541	-	1347	WP_227311888.1	type II toxin-antitoxin system HipA family toxin	Toxin
LIN44_RS09150 (LIN44_09150)	1951544..1951876	-	333	WP_227311889.1	helix-turn-helix transcriptional regulator	Antitoxin
LIN44_RS09155 (LIN44_09155)	1952105..1952815	-	711	WP_227311890.1	VTT domain-containing protein	-
LIN44_RS09160 (LIN44_09160)	1953044..1953895	+	852	WP_227311891.1	LuxR C-terminal-related transcriptional regulator	-
LIN44_RS09165 (LIN44_09165)	1953926..1955155	-	1230	WP_227311892.1	hypothetical protein	-
LIN44_RS09170 (LIN44_09170)	1955454..1955975	-	522	WP_227311893.1	tail fiber protein	-
LIN44_RS09175 (LIN44_09175)	1956012..1956548	-	537	WP_227311894.1	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 449 a.a.
• Molecular weight: 49318.20 Da
• Isoelectric Point: 8.7950

>T220725 WP_227311888.1 NZ_CP085344:c1951541-1950195 [Cupriavidus sp. MP-37]
MARARQGNRLDLWMNGIPVGHWETGAGGDRLVYREDWIADPQGRPLSLSLPFTPGNQPHRGPAVANYFDNLLPDSDVIRR
RIAARYRAGGTDAFALLARVGRDCVGAIQMLPPGEAPEALQTVSGRALSDEDIATLLRETTSAPVLGQREPIDDLRLSIA
GAQEKTALLRWRGQWQLPQGSTPTTHIFKLPLGLVGNMRADMRTSVENEWLCAQIVAAYGLPVARCEIARFADTKALVVE
RFDRRPSSRGTWLLRLPQEDMCQATGISALQKYESDGGPGIQPIAEILSGSRAAATDRRHFFMAQVVFWLLAATDGHAKN
FSISHLPGSQYEATPLYDVLSAHPVIGRGRNQLAPQKARLAMALHGRNRHYAIAEIRRRHWFAQGQRIGFPPAEVEAILD
HVTGLTAQVIDKVASGLPAEFPLDLADAIFDGMRRHNRRLATPAETDL

Nucleotide

• Length: 1347 bp

>T220725 NZ_CP117261:398681-399088 [Rhizobium tumorigenes]
GTGACCACCGCCGCTACCAACTTCCTCGACACAAACATTCTGATCTATGCGATTTCGGACGATAGACGCGCCATTCAGGC
GCAGGCCCTTCTTGACCTCCCTTTTGTGGTCAGTGGCCAGACGCTCAACGAGTTCGCCAACGTAGCACGCAAGAAGCTTT
CCATGTCCTGGCAGGACACCAGCACTGCAATCGAAGCCATTGTCTCGATTTCGACGCTCGTTGTTCCAGTCGACGAGAAG
GTCACGCTCGCCGCCCTCAAACTCGCTCCGCTCTACAATCTCTCATTTTACGACGCGGCCATGATAGCTGCTGCGCTGCA
GGCTGGCTGCAAGCAATATTATTCCGAAGATATGCAGCATGGCCTGGTTGTCGAAAAGCATCTGACGATAGTCAATCCGT
TTCACTAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12197.09 Da
• Isoelectric Point: 11.3844

>AT220725 WP_227311889.1 NZ_CP085344:c1951876-1951544 [Cupriavidus sp. MP-37]
MHYAIKTVHQLRPILQGFRKSRGMTQAVMAEHLGVTQQTYAELEANPAAVSVERLLRVLRVLGVELVLSQAEAGDTAQPV
PARPRAKAKAPASPPEQARPATRRRPREDW

Nucleotide

• Length: 333 bp

>AT220725 NZ_CP117261:398454-398684 [Rhizobium tumorigenes]
ATGCAAGTTGCAAAATGGGGAAACAGCCTTGCCATTCGTATTCCTTCGTCTGTCGTCGATGCCCTCGATCTAAAGGAGGG
TGACGACATTTCGGTGAGCATAAGCAATGATCGCGCCGTCGAAATCGAGCGTGACGATCGACGGCTCAAGGCACTCGAAA
GGATCAAATCCTTCGGCGTCAAGCTGCCGGTAGATTGGAAATTCGATCGTAACGACGCGAACGCGCGGTGA