Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
| Location | 22502..23073 | Replicon | plasmid pEFS108_2 |
| Accession | NZ_CP085296 | ||
| Organism | Enterococcus faecalis strain EFS108 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A2Z2CGM4 |
| Locus tag | LIT97_RS14170 | Protein ID | WP_002362432.1 |
| Coordinates | 22732..23073 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | R3KHK9 |
| Locus tag | LIT97_RS14165 | Protein ID | WP_002362431.1 |
| Coordinates | 22502..22732 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LIT97_RS14110 (LIT97_14110) | 18062..18358 | - | 297 | WP_002367795.1 | hypothetical protein | - |
| LIT97_RS14115 (LIT97_14115) | 18615..18701 | - | 87 | WP_012881081.1 | type I toxin-antitoxin system Fst family toxin | - |
| LIT97_RS14120 (LIT97_14120) | 18793..19008 | - | 216 | WP_002415356.1 | hypothetical protein | - |
| LIT97_RS14125 (LIT97_14125) | 18956..19474 | - | 519 | WP_002367793.1 | hypothetical protein | - |
| LIT97_RS14130 (LIT97_14130) | 19890..20231 | - | 342 | WP_002367792.1 | bacteriocin immunity protein | - |
| LIT97_RS14135 (LIT97_14135) | 20232..20447 | - | 216 | WP_002367791.1 | leucocin A/sakacin P family class II bacteriocin | - |
| LIT97_RS14140 (LIT97_14140) | 20617..20808 | - | 192 | WP_002367788.1 | hypothetical protein | - |
| LIT97_RS14145 (LIT97_14145) | 20820..21014 | - | 195 | WP_002367787.1 | hypothetical protein | - |
| LIT97_RS14150 (LIT97_14150) | 21174..21380 | - | 207 | WP_002367786.1 | hypothetical protein | - |
| LIT97_RS14155 (LIT97_14155) | 21374..21688 | - | 315 | WP_002367785.1 | hypothetical protein | - |
| LIT97_RS14160 (LIT97_14160) | 21678..22298 | - | 621 | WP_002367784.1 | recombinase family protein | - |
| LIT97_RS14165 (LIT97_14165) | 22502..22732 | + | 231 | WP_002362431.1 | hypothetical protein | Antitoxin |
| LIT97_RS14170 (LIT97_14170) | 22732..23073 | + | 342 | WP_002362432.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LIT97_RS14175 (LIT97_14175) | 23185..24123 | + | 939 | WP_002362433.1 | hypothetical protein | - |
| LIT97_RS14180 (LIT97_14180) | 24388..24990 | + | 603 | WP_002362434.1 | Fic family protein | - |
| LIT97_RS14185 (LIT97_14185) | 25129..26267 | + | 1139 | WP_094467193.1 | IS3 family transposase | - |
| LIT97_RS14190 (LIT97_14190) | 26716..27315 | + | 600 | WP_002405175.1 | tyrosine-type recombinase/integrase | - |
| LIT97_RS14195 (LIT97_14195) | 27364..28059 | + | 696 | WP_002405176.1 | Fic family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | tet(M) / tet(L) / cat / erm(B) | - | 1..77870 | 77870 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13244.44 Da Isoelectric Point: 8.0113
>T220718 WP_002362432.1 NZ_CP085296:22732-23073 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T220718 NZ_CP117256:148643-148906 [Rhizobium tumorigenes]
ATGTTCACGATTAGGCGCACCCGTAATTATGCGGACTGGATCAAAACGCTAAAGGACTTTCGTGCCCAAGCTCGAATAGC
CGACCGCATTGACCGTCTCGCCGCAGGAAATCCTGGGGATGTAAAGCCAGTCGGCGAAGGCGTGAGCGAACTCCGCATTA
ACTACGGGCCTGGTTATCGTGTCAATTTTGTCCGGGAAGGGTTGGTCGTCTATGTTCTCCTCTGTCGTGGCGACAAGAGT
ACCCAAGAGAAAGATATTCGATAG
ATGTTCACGATTAGGCGCACCCGTAATTATGCGGACTGGATCAAAACGCTAAAGGACTTTCGTGCCCAAGCTCGAATAGC
CGACCGCATTGACCGTCTCGCCGCAGGAAATCCTGGGGATGTAAAGCCAGTCGGCGAAGGCGTGAGCGAACTCCGCATTA
ACTACGGGCCTGGTTATCGTGTCAATTTTGTCCGGGAAGGGTTGGTCGTCTATGTTCTCCTCTGTCGTGGCGACAAGAGT
ACCCAAGAGAAAGATATTCGATAG
Antitoxin
Download Length: 77 a.a. Molecular weight: 8510.42 Da Isoelectric Point: 4.3855
>AT220718 WP_002362431.1 NZ_CP085296:22502-22732 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
Download Length: 231 bp
>AT220718 NZ_CP117256:148944-149258 [Rhizobium tumorigenes]
ATGCAACTTGAGACATTTGAGTACGACTCCGCCGAATTTCTTGGAAGTGAGGAAGCGATTGCTGAATATCTTTTAGCTGC
TAGCGAAGATGGAGACGCAAACCATATTGCTCGCGCTCTTGGTGTTGTCGCGCGGGCGCGCGGCATGTCCGATCTTTCTC
GGAATACCGGACTTACGCGACCTGCTCTTTACCGGGCAATAAGTGGAGATGGCAATCCAGAGTTTGGGACAATCGCAAAG
GTAGCATACGCACTTGGTTATAGGCTAAATCTCGTTTTGAAATCCGACAGCGGATCGACCGCCGCGGTGAAGTGA
ATGCAACTTGAGACATTTGAGTACGACTCCGCCGAATTTCTTGGAAGTGAGGAAGCGATTGCTGAATATCTTTTAGCTGC
TAGCGAAGATGGAGACGCAAACCATATTGCTCGCGCTCTTGGTGTTGTCGCGCGGGCGCGCGGCATGTCCGATCTTTCTC
GGAATACCGGACTTACGCGACCTGCTCTTTACCGGGCAATAAGTGGAGATGGCAATCCAGAGTTTGGGACAATCGCAAAG
GTAGCATACGCACTTGGTTATAGGCTAAATCTCGTTTTGAAATCCGACAGCGGATCGACCGCCGCGGTGAAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z2CGM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3KHK9 |