Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13645..14782 Replicon plasmid pEFS36_2
Accession NZ_CP085293
Organism Enterococcus faecalis strain EFS36

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag LIT96_RS15115 Protein ID WP_024377178.1
Coordinates 13645..14508 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LIT96_RS15120 Protein ID WP_000301765.1
Coordinates 14510..14782 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT96_RS15080 (LIT96_15080) 8830..9162 - 333 WP_065813813.1 CagC family type IV secretion system protein -
LIT96_RS15085 (LIT96_15085) 9186..11165 - 1980 WP_227316592.1 MobQ family relaxase -
LIT96_RS15090 (LIT96_15090) 11448..11747 + 300 WP_002325624.1 hypothetical protein -
LIT96_RS15095 (LIT96_15095) 11750..12007 + 258 WP_002325623.1 hypothetical protein -
LIT96_RS15100 (LIT96_15100) 12030..12335 - 306 WP_000351790.1 hypothetical protein -
LIT96_RS15105 (LIT96_15105) 12369..12866 - 498 WP_002300565.1 molecular chaperone DnaJ -
LIT96_RS15110 (LIT96_15110) 12886..13203 - 318 WP_002338433.1 hypothetical protein -
LIT96_RS15115 (LIT96_15115) 13645..14508 - 864 WP_024377178.1 zeta toxin family protein Toxin
LIT96_RS15120 (LIT96_15120) 14510..14782 - 273 WP_000301765.1 antitoxin Antitoxin
LIT96_RS15125 (LIT96_15125) 14799..15014 - 216 WP_001835296.1 peptide-binding protein -
LIT96_RS15130 (LIT96_15130) 15114..16010 - 897 WP_024376821.1 ParA family protein -
LIT96_RS15135 (LIT96_15135) 16113..16373 - 261 Protein_22 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LIT96_RS15140 (LIT96_15140) 16543..17280 - 738 WP_065813903.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LIT96_RS15145 (LIT96_15145) 17327..17566 - 240 WP_002425012.1 peptide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / Cfr(D) / poxtA / fexA - 1..35757 35757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32462.96 Da        Isoelectric Point: 6.6610

>T220688 WP_024377178.1 NZ_CP085293:c14508-13645 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T220688 NZ_CP117244:316716-317084 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT220688 WP_000301765.1 NZ_CP085293:c14782-14510 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT220688 NZ_CP117244:316498-316719 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References