Detailed information of TA system TA220683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 932..2040
• Replicon: plasmid pEFS36_1
• Accession: NZ_CP085292
• Organism: Enterococcus faecalis strain EFS36

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: LIT96_RS14790
• Protein ID: WP_071661597.1
• Coordinates: 1171..2040 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LIT96_RS14785
• Protein ID: WP_000205227.1
• Coordinates: 932..1156 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT96_RS14780 (655)	655..789	+	135	Protein_1	site-specific recombinase	-
LIT96_RS14785 (932)	932..1156	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LIT96_RS14790 (1171)	1171..2040	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
LIT96_RS14795 (2021)	2021..2755	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LIT96_RS14800 (2788)	2788..2898	+	111	Protein_5	aminoglycoside 6-adenylyltransferase	-
LIT96_RS14805 (2917)	2917..3309	+	393	WP_000393259.1	GNAT family N-acetyltransferase	-
LIT96_RS14810 (3310)	3310..4749	+	1440	WP_227316581.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
LIT96_RS14815 (4919)	4919..5716	+	798	Protein_8	aminoglycoside 6-adenylyltransferase	-
LIT96_RS14820 (5713)	5713..6255	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III / erm(B) / lsa(E) / lnu(B)	-	1..43405	43405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T220683 WP_071661597.1 NZ_CP085292:1171-2040 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T220683 NZ_CP117242:c2257370-2257266 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT220683 WP_000205227.1 NZ_CP085292:932-1156 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT220683 NZ_CP117242:2257172-2257210 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6