Detailed information of TA system TA220682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 1829354..1830049
• Replicon: chromosome
• Accession: NZ_CP085291
• Organism: Enterococcus faecalis strain EFS36

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: LIT96_RS08840
• Protein ID: WP_002367289.1
• Coordinates: 1829354..1829698 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LIT96_RS08845
• Protein ID: WP_172494175.1
• Coordinates: 1829717..1830049 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT96_RS08820 (1826599)	1826599..1826748	+	150	WP_002356321.1	50S ribosomal protein L33	-
LIT96_RS08825 (1826851)	1826851..1828032	-	1182	WP_172494177.1	site-specific integrase	-
LIT96_RS08830 (1828187)	1828187..1828909	-	723	WP_172494176.1	potassium channel family protein	-
LIT96_RS08835 (1828927)	1828927..1829325	-	399	WP_010706860.1	hypothetical protein	-
LIT96_RS08840 (1829354)	1829354..1829698	-	345	WP_002367289.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LIT96_RS08845 (1829717)	1829717..1830049	-	333	WP_172494175.1	helix-turn-helix transcriptional regulator	Antitoxin
LIT96_RS08850 (1830347)	1830347..1830529	+	183	WP_002358110.1	hypothetical protein	-
LIT96_RS08855 (1830542)	1830542..1830880	+	339	WP_227316466.1	hypothetical protein	-
LIT96_RS08860 (1830943)	1830943..1831122	+	180	WP_227316567.1	DUF2829 domain-containing protein	-
LIT96_RS08865 (1831140)	1831140..1831409	+	270	WP_002367284.1	hypothetical protein	-
LIT96_RS08870 (1831444)	1831444..1831650	+	207	WP_227316467.1	hypothetical protein	-
LIT96_RS08875 (1831689)	1831689..1831988	+	300	WP_172494173.1	hypothetical protein	-
LIT96_RS08880 (1831985)	1831985..1832209	+	225	WP_002367281.1	hypothetical protein	-
LIT96_RS08885 (1832312)	1832312..1832617	+	306	WP_002395805.1	hypothetical protein	-
LIT96_RS08890 (1832636)	1832636..1832956	-	321	WP_010707143.1	hypothetical protein	-
LIT96_RS08895 (1833006)	1833006..1834022	+	1017	WP_227316468.1	RecT family recombinase	-
LIT96_RS08900 (1833985)	1833985..1834800	+	816	WP_172494171.1	PD-(D/E)XK nuclease-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1826851..1869598	42747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13668.65 Da
• Isoelectric Point: 5.2241

>T220682 WP_002367289.1 NZ_CP085291:c1829698-1829354 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T220682 NZ_CP117242:c2200072-2199863 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12733.34 Da
• Isoelectric Point: 5.2423

>AT220682 WP_172494175.1 NZ_CP085291:c1830049-1829717 [Enterococcus faecalis]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKISDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT220682 NZ_CP117242:c2200377-2200084 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA