Detailed information of TA system TA220677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1471242..1471813
• Replicon: chromosome
• Accession: NZ_CP085291
• Organism: Enterococcus faecalis strain EFS36

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LIT96_RS07185
• Protein ID: WP_002354774.1
• Coordinates: 1471472..1471813 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LIT96_RS07180
• Protein ID: WP_002354773.1
• Coordinates: 1471242..1471472 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT96_RS07160 (1467479)	1467479..1468774	+	1296	WP_010815865.1	ATP-binding protein	-
LIT96_RS07165 (1468783)	1468783..1469415	+	633	WP_002358972.1	RloB family protein	-
LIT96_RS07170 (1469482)	1469482..1470474	-	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LIT96_RS07175 (1470613)	1470613..1470828	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LIT96_RS07180 (1471242)	1471242..1471472	+	231	WP_002354773.1	hypothetical protein	Antitoxin
LIT96_RS07185 (1471472)	1471472..1471813	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT96_RS07190 (1472184)	1472184..1475798	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T220677 WP_002354774.1 NZ_CP085291:1471472-1471813 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T220677 NZ_CP117242:c861363-861175 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTATCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT220677 WP_002354773.1 NZ_CP085291:1471242-1471472 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT220677 NZ_CP117242:c861652-861392 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812