Detailed information of TA system TA220669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 911015..911549
• Replicon: chromosome
• Accession: NZ_CP085291
• Organism: Enterococcus faecalis strain EFS36

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: LIT96_RS04400
• Protein ID: WP_002360769.1
• Coordinates: 911015..911290 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: LIT96_RS04405
• Protein ID: WP_002369771.1
• Coordinates: 911283..911549 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT96_RS04370 (906063)	906063..907001	-	939	WP_002370935.1	2-dehydropantoate 2-reductase	-
LIT96_RS04375 (907129)	907129..907458	-	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
LIT96_RS04380 (907488)	907488..907673	+	186	WP_002358660.1	hypothetical protein	-
LIT96_RS04385 (907705)	907705..908313	+	609	Protein_855	IS6 family transposase	-
LIT96_RS04390 (908982)	908982..909956	-	975	WP_002355428.1	choloylglycine hydrolase	-
LIT96_RS04395 (910180)	910180..910824	+	645	WP_002377952.1	IS6 family transposase	-
LIT96_RS04400 (911015)	911015..911290	-	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LIT96_RS04405 (911283)	911283..911549	-	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LIT96_RS04410 (911660)	911660..912244	-	585	WP_002377949.1	thermonuclease family protein	-
LIT96_RS04415 (912268)	912268..912684	-	417	WP_010710134.1	single-stranded DNA-binding protein	-
LIT96_RS04420 (912760)	912760..913008	-	249	WP_002360773.1	DUF3850 domain-containing protein	-
LIT96_RS04425 (913021)	913021..913521	-	501	WP_002360775.1	DnaJ domain-containing protein	-
LIT96_RS04430 (913554)	913554..913820	-	267	WP_002377947.1	hypothetical protein	-
LIT96_RS04435 (914412)	914412..914900	-	489	WP_010710133.1	hypothetical protein	-
LIT96_RS04440 (914906)	914906..915499	-	594	WP_002414802.1	replication-relaxation family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	esp / cylI / cylA / cylB / cylM / cylM / cylS / cylL / cylR1 / cylR2 / bsh / EF0485	826578..936789	110211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T220669 WP_002360769.1 NZ_CP085291:c911290-911015 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T220669 NZ_CP117236:c21863-21582 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATTA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT220669 WP_002369771.1 NZ_CP085291:c911549-911283 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT220669 NZ_CP117236:c22104-21853 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7