Detailed information of TA system TA220665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 30902..32039
• Replicon: plasmid pEFS17_1
• Accession: NZ_CP085290
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LIT95_RS13815
• Protein ID: WP_227264213.1
• Coordinates: 30902..31765 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL19
• Locus tag: LIT95_RS13820
• Protein ID: WP_002326825.1
• Coordinates: 31767..32039 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS13770 (LIT95_13770)	26142..26252	-	111	Protein_36	aminoglycoside 6-adenylyltransferase	-
LIT95_RS13775 (LIT95_13775)	26271..26387	-	117	Protein_37	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LIT95_RS13780 (LIT95_13780)	26557..27294	-	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LIT95_RS13785 (LIT95_13785)	27419..27502	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
LIT95_RS13790 (LIT95_13790)	27625..27792	-	168	Protein_40	peptide-binding protein	-
LIT95_RS13795 (LIT95_13795)	27926..28552	-	627	Protein_41	DNA topoisomerase	-
LIT95_RS13800 (LIT95_13800)	28628..29308	+	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
LIT95_RS13805 (LIT95_13805)	29342..29848	-	507	WP_002415429.1	trimethoprim-resistant dihydrofolate reductase DfrG	-
LIT95_RS13810 (LIT95_13810)	30145..30462	-	318	WP_002326830.1	hypothetical protein	-
LIT95_RS13815 (LIT95_13815)	30902..31765	-	864	WP_227264213.1	zeta toxin family protein	Toxin
LIT95_RS13820 (LIT95_13820)	31767..32039	-	273	WP_002326825.1	antitoxin	Antitoxin
LIT95_RS13825 (LIT95_13825)	32057..32272	-	216	WP_001835296.1	peptide-binding protein	-
LIT95_RS13830 (LIT95_13830)	32364..33260	-	897	WP_002326827.1	ParA family protein	-
LIT95_RS13835 (LIT95_13835)	33363..33623	-	261	Protein_49	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LIT95_RS13840 (LIT95_13840)	33793..34530	-	738	WP_001038795.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LIT95_RS13845 (LIT95_13845)	34655..34738	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
LIT95_RS13850 (LIT95_13850)	35170..35541	-	372	WP_126254895.1	Replication-associated protein RepC	-
LIT95_RS13855 (LIT95_13855)	35534..36379	-	846	WP_000239313.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG / cat / tet(L) / tet(M) / str	-	1..75361	75361
	-	inside	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG	-	10120..34530	24410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32702.34 Da
• Isoelectric Point: 8.2489

>T220665 WP_227264213.1 NZ_CP085290:c31765-30902 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDKFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T220665 NZ_CP117235:c4338077-4337790 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.17 Da
• Isoelectric Point: 4.4865

>AT220665 WP_002326825.1 NZ_CP085290:c32039-31767 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Nucleotide

• Length: 273 bp

>AT220665 NZ_CP117235:c4337803-4337501 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2AF93