Detailed information of TA system TA220664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 14689..15797
• Replicon: plasmid pEFS17_1
• Accession: NZ_CP085290
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: LIT95_RS13705
• Protein ID: WP_078126936.1
• Coordinates: 14689..15558 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: LIT95_RS13710
• Protein ID: WP_002303393.1
• Coordinates: 15573..15797 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS13675 (LIT95_13675)	10120..10857	-	738	WP_001038795.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LIT95_RS13680 (LIT95_13680)	10982..11065	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
LIT95_RS13685 (LIT95_13685)	11607..12401	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LIT95_RS13690 (LIT95_13690)	12494..13036	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LIT95_RS13695 (LIT95_13695)	13033..13941	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LIT95_RS13700 (LIT95_13700)	13974..14708	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LIT95_RS13705 (LIT95_13705)	14689..15558	-	870	WP_078126936.1	nucleotidyltransferase domain-containing protein	Toxin
LIT95_RS13710 (LIT95_13710)	15573..15797	-	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
LIT95_RS13715 (LIT95_13715)	15940..16761	-	822	Protein_25	recombinase family protein	-
LIT95_RS13720 (LIT95_13720)	17449..18252	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LIT95_RS13725 (LIT95_13725)	18306..19790	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
LIT95_RS13730 (LIT95_13730)	20233..20796	-	564	Protein_28	recombinase zinc beta ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG / cat / tet(L) / tet(M) / str	-	1..75361	75361
	-	inside	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG	-	10120..34530	24410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32835.43 Da
• Isoelectric Point: 4.7269

>T220664 WP_078126936.1 NZ_CP085290:c15558-14689 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T220664 NZ_CP117235:4269462-4269614 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT220664 WP_002303393.1 NZ_CP085290:c15797-15573 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT220664 NZ_CP117235:c4269413-4269356 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin

• AlphaFold DB: A0A1M5SHM7