Detailed information of TA system TA220663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 3513..4084
• Replicon: plasmid pEFS17_1
• Accession: NZ_CP085290
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z2CGM4
• Locus tag: LIT95_RS13605
• Protein ID: WP_002362432.1
• Coordinates: 3513..3854 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: LIT95_RS13610
• Protein ID: WP_002362431.1
• Coordinates: 3854..4084 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS13595 (LIT95_13595)	1596..2198	-	603	WP_002362434.1	Fic family protein	-
LIT95_RS13600 (LIT95_13600)	2463..3401	-	939	WP_002362433.1	hypothetical protein	-
LIT95_RS13605 (LIT95_13605)	3513..3854	-	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT95_RS13610 (LIT95_13610)	3854..4084	-	231	WP_002362431.1	hypothetical protein	Antitoxin
LIT95_RS13615 (LIT95_13615)	4288..4908	+	621	WP_002367784.1	recombinase family protein	-
LIT95_RS13620 (LIT95_13620)	4898..5212	+	315	WP_002367785.1	hypothetical protein	-
LIT95_RS13625 (LIT95_13625)	5206..5412	+	207	WP_002367786.1	hypothetical protein	-
LIT95_RS13630 (LIT95_13630)	5572..5766	+	195	WP_002367787.1	hypothetical protein	-
LIT95_RS13635 (LIT95_13635)	5778..5969	+	192	WP_002367788.1	hypothetical protein	-
LIT95_RS13640 (LIT95_13640)	6139..6354	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
LIT95_RS13645 (LIT95_13645)	6355..6696	+	342	WP_002367792.1	bacteriocin immunity protein	-
LIT95_RS13650 (LIT95_13650)	7112..7630	+	519	WP_002367793.1	hypothetical protein	-
LIT95_RS13655 (LIT95_13655)	7578..7793	+	216	WP_002415356.1	hypothetical protein	-
LIT95_RS13660 (LIT95_13660)	7885..7971	+	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
LIT95_RS13665 (LIT95_13665)	8228..8524	+	297	WP_002367795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG / cat / tet(L) / tet(M) / str	-	1..75361	75361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13244.44 Da
• Isoelectric Point: 8.0113

>T220663 WP_002362432.1 NZ_CP085290:c3854-3513 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T220663 NZ_CP117235:c4232527-4232213 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT220663 WP_002362431.1 NZ_CP085290:c4084-3854 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT220663 NZ_CP117235:c4232763-4232530 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A2Z2CGM4

Antitoxin

• AlphaFold DB: R3KHK9