Detailed information of TA system TA220659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 1562468..1563002
• Replicon: chromosome
• Accession: NZ_CP085289
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: LIT95_RS07565
• Protein ID: WP_002360769.1
• Coordinates: 1562727..1563002 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: LIT95_RS07560
• Protein ID: WP_002369771.1
• Coordinates: 1562468..1562734 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS07525 (1558518)	1558518..1559111	+	594	WP_002414802.1	replication-relaxation family protein	-
LIT95_RS07530 (1559117)	1559117..1559605	+	489	WP_010710133.1	hypothetical protein	-
LIT95_RS07535 (1560197)	1560197..1560463	+	267	WP_002377947.1	hypothetical protein	-
LIT95_RS07540 (1560496)	1560496..1560996	+	501	WP_002360775.1	DnaJ domain-containing protein	-
LIT95_RS07545 (1561009)	1561009..1561257	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
LIT95_RS07550 (1561333)	1561333..1561749	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
LIT95_RS07555 (1561773)	1561773..1562357	+	585	WP_002377949.1	thermonuclease family protein	-
LIT95_RS07560 (1562468)	1562468..1562734	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LIT95_RS07565 (1562727)	1562727..1563002	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LIT95_RS07570 (1563193)	1563193..1563837	-	645	WP_002289351.1	IS6 family transposase	-
LIT95_RS07575 (1564061)	1564061..1565035	+	975	WP_002355428.1	choloylglycine hydrolase	-
LIT95_RS07580 (1565704)	1565704..1566312	-	609	Protein_1467	IS6 family transposase	-
LIT95_RS07585 (1566344)	1566344..1566529	-	186	WP_002358660.1	hypothetical protein	-
LIT95_RS07590 (1566559)	1566559..1566888	+	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
LIT95_RS07595 (1567016)	1567016..1567954	+	939	WP_002370935.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	EF0485 / bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / esp	1537093..1640875	103782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T220659 WP_002360769.1 NZ_CP085289:1562727-1563002 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T220659 NZ_CP117235:2998687-2998965 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT220659 WP_002369771.1 NZ_CP085289:1562468-1562734 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT220659 NZ_CP117235:2998494-2998685 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7