Detailed information of TA system TA220649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1050500..1051071
• Replicon: chromosome
• Accession: NZ_CP085289
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LIT95_RS05040
• Protein ID: WP_002396016.1
• Coordinates: 1050500..1050841 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S4CGQ1
• Locus tag: LIT95_RS05045
• Protein ID: WP_002367500.1
• Coordinates: 1050841..1051071 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS05035 (1046515)	1046515..1050129	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LIT95_RS05040 (1050500)	1050500..1050841	-	342	WP_002396016.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LIT95_RS05045 (1050841)	1050841..1051071	-	231	WP_002367500.1	hypothetical protein	Antitoxin
LIT95_RS05050 (1051484)	1051484..1051699	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LIT95_RS05055 (1051838)	1051838..1052830	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LIT95_RS05060 (1052997)	1052997..1053635	-	639	WP_126254829.1	lytic polysaccharide monooxygenase	-
LIT95_RS05065 (1054322)	1054322..1055938	+	1617	WP_126254830.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13165.46 Da
• Isoelectric Point: 9.4141

>T220649 WP_002396016.1 NZ_CP085289:c1050841-1050500 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQQRRLGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDVIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T220649 NZ_CP117235:c830718-830341 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.69 Da
• Isoelectric Point: 4.3313

>AT220649 WP_002367500.1 NZ_CP085289:c1051071-1050841 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT220649 NZ_CP117235:c831175-830795 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: S4CGQ1