Detailed information of TA system TA220641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 675216..675902
• Replicon: chromosome
• Accession: NZ_CP085289
• Organism: Enterococcus faecalis strain EFS17

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: LIT95_RS03275
• Protein ID: WP_114679421.1
• Coordinates: 675558..675902 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LIT95_RS03270
• Protein ID: WP_174089957.1
• Coordinates: 675216..675539 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT95_RS03220 (670735)	670735..671550	-	816	WP_162781818.1	PD-(D/E)XK nuclease-like domain-containing protein	-
LIT95_RS03225 (671513)	671513..672523	-	1011	WP_114679414.1	RecT family recombinase	-
LIT95_RS03230 (672624)	672624..672848	-	225	WP_002367281.1	hypothetical protein	-
LIT95_RS03235 (672845)	672845..673144	-	300	WP_114679415.1	hypothetical protein	-
LIT95_RS03240 (673211)	673211..673390	-	180	WP_114679416.1	hypothetical protein	-
LIT95_RS03245 (673425)	673425..673694	-	270	WP_002367284.1	hypothetical protein	-
LIT95_RS03250 (673770)	673770..674084	+	315	WP_114679417.1	hypothetical protein	-
LIT95_RS14085 (674065)	674065..674193	-	129	WP_114679418.1	hypothetical protein	-
LIT95_RS03255 (674209)	674209..674385	-	177	WP_002385636.1	helix-turn-helix transcriptional regulator	-
LIT95_RS03260 (674473)	674473..674751	+	279	WP_114679419.1	hypothetical protein	-
LIT95_RS03265 (674748)	674748..674909	-	162	WP_174089958.1	hypothetical protein	-
LIT95_RS03270 (675216)	675216..675539	+	324	WP_174089957.1	helix-turn-helix transcriptional regulator	Antitoxin
LIT95_RS03275 (675558)	675558..675902	+	345	WP_114679421.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LIT95_RS03280 (675961)	675961..676689	+	729	WP_114679422.1	potassium channel family protein	-
LIT95_RS03285 (676844)	676844..678025	+	1182	WP_114679423.1	site-specific integrase	-
LIT95_RS03290 (678128)	678128..678277	-	150	WP_002356321.1	50S ribosomal protein L33	-
LIT95_RS03295 (678403)	678403..680538	-	2136	WP_002359399.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	585515..678025	92510
	-	inside	Prophage	-	-	601493..678025	76532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13608.64 Da
• Isoelectric Point: 5.6177

>T220641 WP_114679421.1 NZ_CP085289:675558-675902 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNIIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T220641 NZ_CP117230:c2044970-2044653 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATATGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAATTAGAAAAAGCATCATCTGAGAACTTTGACGAGGAATCAGATGACGCGCAGAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12292.87 Da
• Isoelectric Point: 5.2520

>AT220641 WP_174089957.1 NZ_CP085289:675216-675539 [Enterococcus faecalis]
MTVFDRVKKLADSQKISIVELEEKLNFSRNSLYAWKKSKPSIDKLEAVANYFGVSTDYLLGREVSNKSKQSDDLDEVLDN
VMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 324 bp

>AT220641 NZ_CP117230:c2045182-2044973 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA