Detailed information of TA system TA220640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1250262..1251179
• Replicon: chromosome
• Accession: NZ_CP085283
• Organism: Bacillus velezensis strain TPS3N

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: LJA38_RS06400
• Protein ID: WP_003154806.1
• Coordinates: 1250433..1251179 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LJA38_RS06395
• Protein ID: WP_003154807.1
• Coordinates: 1250262..1250432 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJA38_RS06355	1245493..1247115	+	1623	WP_003154821.1	pyocin knob domain-containing protein	-
LJA38_RS06360	1247128..1247499	+	372	WP_014304856.1	XkdW family protein	-
LJA38_RS06365	1247505..1247702	+	198	WP_003154819.1	XkdX family protein	-
LJA38_RS06370	1247759..1248520	+	762	WP_063174221.1	hypothetical protein	-
LJA38_RS06375	1248572..1248835	+	264	WP_032866112.1	hemolysin XhlA family protein	-
LJA38_RS06380	1248849..1249112	+	264	WP_003154813.1	phage holin	-
LJA38_RS06385	1249126..1250004	+	879	WP_058905962.1	N-acetylmuramoyl-L-alanine amidase	-
LJA38_RS06390	1250040..1250165	-	126	WP_003154809.1	hypothetical protein	-
LJA38_RS06395	1250262..1250432	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LJA38_RS06400	1250433..1251179	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LJA38_RS06405	1251284..1252282	-	999	WP_003154805.1	inorganic phosphate transporter	-
LJA38_RS06410	1252295..1252912	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LJA38_RS06415	1253198..1254514	-	1317	WP_007610842.1	amino acid permease	-
LJA38_RS06420	1254837..1255787	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T220640 WP_003154806.1 NZ_CP085283:c1251179-1250433 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T220640 NZ_CP117230:c1929608-1929456 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT220640 WP_003154807.1 NZ_CP085283:c1250432-1250262 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT220640 NZ_CP117230:c1930232-1929633 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14