Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1250262..1251179 Replicon chromosome
Accession NZ_CP085283
Organism Bacillus velezensis strain TPS3N

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag LJA38_RS06400 Protein ID WP_003154806.1
Coordinates 1250433..1251179 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag LJA38_RS06395 Protein ID WP_003154807.1
Coordinates 1250262..1250432 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJA38_RS06355 1245493..1247115 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
LJA38_RS06360 1247128..1247499 + 372 WP_014304856.1 XkdW family protein -
LJA38_RS06365 1247505..1247702 + 198 WP_003154819.1 XkdX family protein -
LJA38_RS06370 1247759..1248520 + 762 WP_063174221.1 hypothetical protein -
LJA38_RS06375 1248572..1248835 + 264 WP_032866112.1 hemolysin XhlA family protein -
LJA38_RS06380 1248849..1249112 + 264 WP_003154813.1 phage holin -
LJA38_RS06385 1249126..1250004 + 879 WP_058905962.1 N-acetylmuramoyl-L-alanine amidase -
LJA38_RS06390 1250040..1250165 - 126 WP_003154809.1 hypothetical protein -
LJA38_RS06395 1250262..1250432 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
LJA38_RS06400 1250433..1251179 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
LJA38_RS06405 1251284..1252282 - 999 WP_003154805.1 inorganic phosphate transporter -
LJA38_RS06410 1252295..1252912 - 618 WP_003154804.1 DUF47 domain-containing protein -
LJA38_RS06415 1253198..1254514 - 1317 WP_007610842.1 amino acid permease -
LJA38_RS06420 1254837..1255787 + 951 WP_012117369.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T220640 WP_003154806.1 NZ_CP085283:c1251179-1250433 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T220640 NZ_CP117230:c1929608-1929456 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT220640 WP_003154807.1 NZ_CP085283:c1250432-1250262 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT220640 NZ_CP117230:c1930232-1929633 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References