Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 507168..507805 Replicon chromosome
Accession NZ_CP085283
Organism Bacillus velezensis strain TPS3N

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LJA38_RS02455 Protein ID WP_003156187.1
Coordinates 507455..507805 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LJA38_RS02450 Protein ID WP_003156188.1
Coordinates 507168..507449 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJA38_RS02430 503533..504132 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
LJA38_RS02435 504225..504590 + 366 WP_003156192.1 holo-ACP synthase -
LJA38_RS02440 504755..505762 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
LJA38_RS02445 505879..507048 + 1170 WP_003156189.1 alanine racemase -
LJA38_RS02450 507168..507449 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LJA38_RS02455 507455..507805 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LJA38_RS02460 507923..508744 + 822 WP_014304404.1 STAS domain-containing protein -
LJA38_RS02465 508749..509114 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
LJA38_RS02470 509117..509518 + 402 WP_003156178.1 anti-sigma regulatory factor -
LJA38_RS02475 509530..510537 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
LJA38_RS02480 510601..510930 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LJA38_RS02485 510927..511409 + 483 WP_003156173.1 anti-sigma B factor RsbW -
LJA38_RS02490 511375..512163 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LJA38_RS02495 512163..512765 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T220639 WP_003156187.1 NZ_CP085283:507455-507805 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T220639 NZ_CP117230:c820691-820503 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT220639 WP_003156188.1 NZ_CP085283:507168-507449 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT220639 NZ_CP117230:c820980-820720 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References