Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1488717..1489354 Replicon chromosome
Accession NZ_CP085282
Organism Bacillus amyloliquefaciens strain TPS17

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LJA36_RS06930 Protein ID WP_003156187.1
Coordinates 1488717..1489067 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LJA36_RS06935 Protein ID WP_003156188.1
Coordinates 1489073..1489354 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJA36_RS06890 1483757..1484359 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
LJA36_RS06895 1484359..1485147 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LJA36_RS06900 1485113..1485595 - 483 WP_007609591.1 anti-sigma B factor RsbW -
LJA36_RS06905 1485592..1485921 - 330 WP_033575044.1 anti-sigma factor antagonist -
LJA36_RS06910 1485985..1486992 - 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
LJA36_RS06915 1487004..1487405 - 402 WP_003156178.1 anti-sigma regulatory factor -
LJA36_RS06920 1487408..1487773 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
LJA36_RS06925 1487778..1488599 - 822 WP_033575045.1 STAS domain-containing protein -
LJA36_RS06930 1488717..1489067 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LJA36_RS06935 1489073..1489354 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LJA36_RS06940 1489474..1490643 - 1170 WP_040238530.1 alanine racemase -
LJA36_RS06945 1490760..1491767 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
LJA36_RS06950 1491932..1492297 - 366 WP_007410229.1 holo-ACP synthase -
LJA36_RS06955 1492390..1492989 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T220638 WP_003156187.1 NZ_CP085282:c1489067-1488717 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T220638 NZ_CP117230:363797-364120 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGA
GTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT220638 WP_003156188.1 NZ_CP085282:c1489354-1489073 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT220638 NZ_CP117230:363585-363794 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGTGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References