Detailed information of TA system TA220637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 756048..756965
• Replicon: chromosome
• Accession: NZ_CP085282
• Organism: Bacillus amyloliquefaciens strain TPS17

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: LJA36_RS02970
• Protein ID: WP_007407256.1
• Coordinates: 756048..756794 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LJA36_RS02975
• Protein ID: WP_003154807.1
• Coordinates: 756795..756965 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJA36_RS02950	751441..752391	-	951	WP_012117369.1	ring-cleaving dioxygenase	-
LJA36_RS02955	752714..754030	+	1317	WP_007610842.1	amino acid permease	-
LJA36_RS02960	754316..754933	+	618	WP_003154804.1	DUF47 domain-containing protein	-
LJA36_RS02965	754946..755944	+	999	WP_003154805.1	inorganic phosphate transporter	-
LJA36_RS02970	756048..756794	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LJA36_RS02975	756795..756965	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LJA36_RS02980	757062..757187	+	126	WP_023356844.1	hypothetical protein	-
LJA36_RS02985	757223..758101	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
LJA36_RS02990	758115..758378	-	264	WP_003154813.1	phage holin	-
LJA36_RS02995	758392..758655	-	264	WP_003154815.1	hemolysin XhlA family protein	-
LJA36_RS03000	758707..759468	-	762	WP_033575307.1	hypothetical protein	-
LJA36_RS03005	759525..759722	-	198	WP_012117366.1	XkdX family protein	-
LJA36_RS03010	759727..760098	-	372	WP_015417295.1	XkdW family protein	-
LJA36_RS03015	760111..761742	-	1632	WP_015417294.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T220637 WP_007407256.1 NZ_CP085282:756048-756794 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T220637 NZ_CP117229:864341-864688 [Listeria innocua]
CTGATGGTGAAGCGTGGTGACGTTTACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGTAATAGATTCAGTCCAACTGTGATCGTGGCAGCAATAACTGCAAAAATTCAAAAAG
CGAAGTTGCCAACACATGTGGAAGCTACTCGTAAAGACGGCTTTGAAAGAGATTCTGTCATTCTATTAGAACAAATTAGA
ACGATAGATAAACAACGCCTGACAGATAAGATTACACATTTGGACGAGGAACTAATGGCCAAGGTAAATCAGGCACTTGA
AGTCAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT220637 WP_003154807.1 NZ_CP085282:756795-756965 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT220637 NZ_CP117229:864059-864337 [Listeria innocua]
GTGTTAGAGAAAGAAAAACGGATGATAATATCCGTAGAACTGACACAGGAAATGGTACAAGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTACAGGAGAAAAGAGCTCGTGAATTAA
GAGACGAGATGGAACGCGGCTATGCAGAAATGGCGACGATTAATTTCGCTATCGCGTGCGAGTGTACTCATGTCGAAGCA
GAGGCAGAAGACAGGAATATTAGTATTTTAGGAGGTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14