Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 85540..86297 Replicon plasmid p001
Accession NZ_CP085203
Organism Shigella flexneri strain P099

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag IUX27_RS23745 Protein ID WP_000501974.1
Coordinates 85540..86025 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag IUX27_RS23750 Protein ID WP_011114751.1
Coordinates 86013..86297 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUX27_RS23715 (IUX27_23740) 81065..81488 - 424 Protein_107 hypothetical protein -
IUX27_RS23720 (IUX27_23745) 81716..82354 - 639 WP_000502854.1 ParB N-terminal domain-containing protein -
IUX27_RS23725 (IUX27_23750) 82339..82482 - 144 Protein_109 DUF3440 domain-containing protein -
IUX27_RS23740 (IUX27_23765) 85086..85367 + 282 Protein_112 IS4/IS5 family transposase -
IUX27_RS23745 (IUX27_23770) 85540..86025 - 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
IUX27_RS23750 (IUX27_23775) 86013..86297 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
IUX27_RS23755 (IUX27_23780) 87199..87363 + 165 WP_001346193.1 hypothetical protein -
IUX27_RS23760 (IUX27_23785) 87617..89218 - 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
IUX27_RS23765 (IUX27_23790) 89215..89565 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
IUX27_RS23770 (IUX27_23795) 89562..90236 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..206206 206206
- inside IScluster/Tn - ospE2 / ipaH2.5 / ospC2 / ospD3/senA / ospC1 / ospC3 46116..98227 52111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T220633 WP_000501974.1 NZ_CP085203:c86025-85540 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T220633 NZ_CP117227:c4874737-4874453 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT220633 WP_011114751.1 NZ_CP085203:c86297-86013 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT220633 NZ_CP117227:c4874969-4874727 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References