Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4315881..4316512 Replicon chromosome
Accession NZ_CP085202
Organism Shigella flexneri strain P099

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag IUX27_RS21870 Protein ID WP_001259384.1
Coordinates 4316237..4316512 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IUX27_RS21865 Protein ID WP_000593555.1
Coordinates 4315881..4316240 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUX27_RS21840 (4312013) 4312013..4312957 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
IUX27_RS21845 (4312954) 4312954..4313787 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
IUX27_RS21850 (4313787) 4313787..4314551 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
IUX27_RS21855 (4314548) 4314548..4315354 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
IUX27_RS21860 (4315360) 4315360..4315761 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IUX27_RS21865 (4315881) 4315881..4316240 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IUX27_RS21870 (4316237) 4316237..4316512 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
IUX27_RS21875 (4316571) 4316571..4317695 - 1125 WP_001216257.1 ABC transporter permease -
IUX27_RS21880 (4317695) 4317695..4320430 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
IUX27_RS21885 (4320427) 4320427..4321494 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T220631 WP_001259384.1 NZ_CP085202:c4316512-4316237 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T220631 NZ_CP117227:4149331-4149549 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT220631 WP_000593555.1 NZ_CP085202:c4316240-4315881 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT220631 NZ_CP117227:4148930-4149304 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References