Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | itaRT/DUF1778(antitoxin) |
| Location | 3535093..3535937 | Replicon | chromosome |
| Accession | NZ_CP085202 | ||
| Organism | Shigella flexneri strain P099 | ||
Toxin (Protein)
| Gene name | itaT | Uniprot ID | - |
| Locus tag | IUX27_RS17920 | Protein ID | WP_014532172.1 |
| Coordinates | 3535476..3535937 (+) | Length | 154 a.a. |
Antitoxin (Protein)
| Gene name | itaR | Uniprot ID | A5H0K0 |
| Locus tag | IUX27_RS17915 | Protein ID | WP_005053053.1 |
| Coordinates | 3535093..3535404 (+) | Length | 104 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IUX27_RS17895 (3531728) | 3531728..3532342 | + | 615 | WP_000179819.1 | cytochrome o ubiquinol oxidase subunit III | - |
| IUX27_RS17900 (3532342) | 3532342..3532671 | + | 330 | WP_000019869.1 | cytochrome o ubiquinol oxidase subunit IV | - |
| IUX27_RS17905 (3532683) | 3532683..3533573 | + | 891 | WP_000971346.1 | heme o synthase | - |
| IUX27_RS17915 (3535093) | 3535093..3535404 | + | 312 | WP_005053053.1 | DUF1778 domain-containing protein | Antitoxin |
| IUX27_RS17920 (3535476) | 3535476..3535937 | + | 462 | WP_014532172.1 | GNAT family N-acetyltransferase | Toxin |
| IUX27_RS17930 (3536833) | 3536833..3538986 | + | 2154 | WP_005060811.1 | SEL1-like repeat protein | - |
| IUX27_RS17935 (3539039) | 3539039..3539677 | + | 639 | Protein_3481 | hypothetical protein | - |
| IUX27_RS17940 (3539731) | 3539731..3540428 | + | 698 | WP_223368647.1 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | IScluster/Tn | - | - | 3527241..3540428 | 13187 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 154 a.a. Molecular weight: 16623.52 Da Isoelectric Point: 9.9538
>T220629 WP_014532172.1 NZ_CP085202:3535476-3535937 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA
Download Length: 462 bp
>T220629 NZ_CP117227:1982152-1982254 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 104 a.a. Molecular weight: 11394.07 Da Isoelectric Point: 10.3388
>AT220629 WP_005053053.1 NZ_CP085202:3535093-3535404 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA
Download Length: 312 bp
>AT220629 NZ_CP117227:c1982261-1982116 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|