Detailed information of TA system TA220610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 705780..706419
• Replicon: chromosome
• Accession: NZ_CP085200
• Organism: Mycobacterium ulcerans strain JKD8049

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LH162_RS03385
• Protein ID: WP_240168794.1
• Coordinates: 705973..706419 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: B2HEJ8
• Locus tag: LH162_RS03380
• Protein ID: WP_011738542.1
• Coordinates: 705780..705968 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH162_RS03360 (LH162_03360)	701381..702682	+	1302	WP_240168791.1	ISAs1 family transposase	-
LH162_RS03365 (LH162_03365)	702700..702933	-	234	Protein_659	IS256 family transposase	-
LH162_RS03370 (LH162_03370)	702990..704339	-	1350	WP_240168792.1	IS256 family transposase	-
LH162_RS03375 (LH162_03375)	704579..705604	+	1026	WP_240168793.1	hypothetical protein	-
LH162_RS03380 (LH162_03380)	705780..705968	+	189	WP_011738542.1	antitoxin	Antitoxin
LH162_RS03385 (LH162_03385)	705973..706419	+	447	WP_240168794.1	SRPBCC family protein	Toxin
LH162_RS03390 (LH162_03390)	706531..707175	-	645	WP_231507106.1	hypothetical protein	-
LH162_RS03395 (LH162_03395)	707256..707774	-	519	WP_240168795.1	EspA/EspE family type VII secretion system effector	-
LH162_RS03400 (LH162_03400)	707952..708725	+	774	WP_011738540.1	VOC family protein	-
LH162_RS03405 (LH162_03405)	708796..709230	+	435	WP_011738539.1	hypothetical protein	-
LH162_RS03410 (LH162_03410)	709236..709709	-	474	WP_071498317.1	VOC family protein	-
LH162_RS03415 (LH162_03415)	709726..711246	-	1521	WP_240168796.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15860.44 Da
• Isoelectric Point: 9.7664

>T220610 WP_240168794.1 NZ_CP085200:705973-706419 [Mycobacterium ulcerans]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLPETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEQGSIVSFDVHLGGPALFGPIGMVVAAALRSDINASLRNFVTVFAPPAAG

Nucleotide

• Length: 447 bp

>T220610 NZ_CP117201:132080-132424 [Pantoea sp. SS70]
ATGCACGCGACATTTATTGAGCTTTCCAGCTTTCAGAAATACCGGGCTGACTACCTGAACGACGATCAGTTCAGGTTGTT
TCAGAACATGTTGCTGGCTGACCCCGAAAAGGGCGACGTGATCCCGGATACGGGTGGCCTGCGTAAAGTCCGGTTCAGGG
ACGAGCGCCGGAACAAAGGCACACGAGGCGGGATCAGGGTTATCTATTACTGGACGAACGAAAAAGGCCAGTTCATCCTG
TTTACCATCTACGATAAGGATCAACGCGACGATCTGACCAAACAGCAGCGCGACGCCCTGGCGGAGGCGCTTAACGTGAT
TAAAAAGGGGCTGAGACATGACTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6586.35 Da
• Isoelectric Point: 4.7205

>AT220610 WP_011738542.1 NZ_CP085200:705780-705968 [Mycobacterium ulcerans]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT220610 NZ_CP117201:132417-132731 [Pantoea sp. SS70]
ATGACTGAACGTGACATTTTCAGTGAGCTGATGACTGGCATGGAGGAGCTGAAAGATCACCAGGACGGGAAAATCACGCT
CAAGACTTACAAGGTCAGCAAGCGCGCACCCGTCACTATCGAGCCGCAGGAGCTGCGCGACGTGCGCGAAAAACTTAACC
TGTCACAGGCGGTTTTCGCACACTATCTGCATACGGGCGAGACCACCTATCAGAACTGGGAGCAGGGCAGAGCTAAGCCT
AACGCTCAGGCCGTCCTGCTGATCCGCATGGTTCAGCAGAACCCGGACACGCTGCGGACGCTCGCGCAGCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LB02