Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17451..18094 Replicon plasmid unnamed1
Accession NZ_CP085198
Organism Klebsiella quasipneumoniae strain NDM-101

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag LH783_RS25245 Protein ID WP_021312475.1
Coordinates 17451..17867 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag LH783_RS25250 Protein ID WP_021312476.1
Coordinates 17864..18094 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH783_RS25195 (LH783_25225) 12927..13181 - 255 WP_032731922.1 hypothetical protein -
LH783_RS25200 (LH783_25230) 13371..14339 + 969 WP_101991433.1 IS5-like element IS903 family transposase -
LH783_RS25205 (LH783_25235) 14395..14580 - 186 Protein_15 hypothetical protein -
LH783_RS25210 (LH783_25240) 14629..14832 - 204 WP_004150739.1 HHA domain-containing protein -
LH783_RS25215 (LH783_25245) 14893..15387 - 495 WP_016528789.1 hypothetical protein -
LH783_RS25220 (LH783_25250) 15418..15984 - 567 WP_009654312.1 hypothetical protein -
LH783_RS25225 (LH783_25255) 15981..16244 - 264 WP_004118691.1 hypothetical protein -
LH783_RS25230 (LH783_25260) 16484..16810 - 327 WP_124989605.1 hypothetical protein -
LH783_RS25235 (LH783_25265) 16889..17107 - 219 WP_021312527.1 hypothetical protein -
LH783_RS25240 (LH783_25270) 17171..17416 - 246 WP_065905238.1 hypothetical protein -
LH783_RS25245 (LH783_25275) 17451..17867 - 417 WP_021312475.1 type II toxin-antitoxin system VapC family toxin Toxin
LH783_RS25250 (LH783_25280) 17864..18094 - 231 WP_021312476.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LH783_RS25255 (LH783_25285) 18699..18992 + 294 WP_124989603.1 hypothetical protein -
LH783_RS25260 (LH783_25290) 19041..19514 + 474 WP_065905236.1 hypothetical protein -
LH783_RS25265 (LH783_25295) 19516..20484 + 969 WP_078207718.1 IS5 family transposase -
LH783_RS25270 (LH783_25300) 21171..21971 - 801 WP_065905270.1 EAL domain-containing protein -
LH783_RS25275 (LH783_25305) 22023..22751 - 729 Protein_29 CSS-motif domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..183890 183890
- flank IS/Tn - - 19516..20484 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15033.44 Da        Isoelectric Point: 7.8915

>T220608 WP_021312475.1 NZ_CP085198:c17867-17451 [Klebsiella quasipneumoniae]
VKKTFMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T220608 NZ_CP117200:407127-407471 [Pantoea sp. SS70]
ATGCACGCGACATTTATTGAGCTGTCCAGCTTTCAGAAGTACCGGGTGGAATACCTGAGCGATGATCAGTTTAGGCTTTT
CCAGAATATGTTGATGGCCGACCCTGAAAAGGGCGACGTGATCCCAGATACGGGCGGCCTGCGTAAAGTCCGGTTCAGGG
ATGAGCGTCGAAACAAGGGCACAAGAGGCGGCATTAGGGTTATCTATTACTGGACGAACGAAAAAGGCCAGTTCATCCTG
TTTACCATTTACGATAAGGATCAACGCGACGATCTGACCAAACAGCAGCGCGACGCCTTGGGTTCGGCGCTGAACGTGAT
TAAAAAGGGGCTGAGACATGACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT220608 WP_021312476.1 NZ_CP085198:c18094-17864 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT220608 NZ_CP117200:407464-407778 [Pantoea sp. SS70]
ATGACTGAACGCGATATTTTTAGTGAGCTGATGACGGGAATGCAGGAGCTGAAACATCATAAGGACGGAAAAATCACGCT
CAAGACCTACAAGGTCAGCAAGCGCGCGCCCGTCACCATCGCGCCACAGGAGCTGCGTGATGTACGCGAAAAACTTAACC
TTTCTCAGGCCGTTTTTGCGCACTACCTTCACACAGGTGAAACCACCTATCAAAACTGGGAGCAAGGCAGGTCCAGGCCT
AACGCCCAGGCTGTTTTGCTTATACGCATGGTTCAGCAGAATCCTGAAACCTTGAAAACGCTCGCACAGCTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References