Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4588439..4589058 Replicon chromosome
Accession NZ_CP085197
Organism Klebsiella quasipneumoniae strain NDM-101

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LH783_RS22185 Protein ID WP_002892050.1
Coordinates 4588840..4589058 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LH783_RS22180 Protein ID WP_002892066.1
Coordinates 4588439..4588813 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH783_RS22170 (4583592) 4583592..4584785 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LH783_RS22175 (4584808) 4584808..4587954 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit AcrB -
LH783_RS22180 (4588439) 4588439..4588813 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LH783_RS22185 (4588840) 4588840..4589058 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LH783_RS22190 (4589216) 4589216..4589782 + 567 WP_004204750.1 maltose O-acetyltransferase -
LH783_RS22195 (4589754) 4589754..4589876 - 123 WP_032426076.1 hypothetical protein -
LH783_RS22200 (4589919) 4589919..4590389 + 471 WP_004204751.1 YlaC family protein -
LH783_RS22205 (4590358) 4590358..4591815 - 1458 WP_065903537.1 PLP-dependent aminotransferase family protein -
LH783_RS22210 (4591916) 4591916..4592614 + 699 WP_064187826.1 GNAT family protein -
LH783_RS22215 (4592611) 4592611..4592751 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LH783_RS22220 (4592751) 4592751..4593014 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220607 WP_002892050.1 NZ_CP085197:4588840-4589058 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T220607 NZ_CP117200:c356341-355964 [Pantoea sp. SS70]
ATGATTCACGTTTCTGCCGAGGAGGTTATCGCACTTCACGATTACCTTTTAAAGCGTTATGCCGGTGTTGCAGGAATGTC
CGATCCCGGAAGAGCAGAAGCTATTGTTGCCCGCGTTATTAATCGTGAATATTACGAAGGGATTCAGGATATTTTTGAGC
TTGCAGCAACCTATTGGGTGGCAATCTCACGCGGGCATATTTTTGCCGATGCGAATAAGAGAACCTCGCTTAACGTAACC
ATGTTGTTTTTAAAGCGTAATGGAATACGCGTACATGACCGGCCTGAATTAGTCGAATTGACGGTCATGGCAGCAACTGG
CGAAGCCGGAGTGCCTTATCTGGCAAACCAATTGCGTGCATTCTTCGGATCACACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT220607 WP_002892066.1 NZ_CP085197:4588439-4588813 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT220607 NZ_CP117200:c356559-356338 [Pantoea sp. SS70]
ATGAACAAGTTTACTTATACCAGCGCCAGAGAGAATTTATCTCAGGTTTTGGACCAGGCGATTGCAGGCGAGGCCGTTGA
AATTACTCGACGCAGCGGTGACTCAGCGGTTGTCATCAGCAAAGCCAGTTTCGAGGCGTATCGAAAAGCCGTACTGGATG
CTGAATTCGCAGAGTTAATGGGTGAGTTTGATGCATCTAATAAAGCGTTAACAGATAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References