Detailed information of TA system TA220606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3266549..3268137
• Replicon: chromosome
• Accession: NZ_CP085197
• Organism: Klebsiella quasipneumoniae strain NDM-101

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LH783_RS15810
• Protein ID: WP_064187239.1
• Coordinates: 3266549..3267871 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: LH783_RS15815
• Protein ID: WP_004205921.1
• Coordinates: 3267871..3268137 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH783_RS15775 (3261631)	3261631..3261849	+	219	WP_002904808.1	ParD-like family protein	-
LH783_RS15780 (3261830)	3261830..3262612	+	783	WP_065904745.1	type I methionyl aminopeptidase	-
LH783_RS15785 (3262881)	3262881..3263996	+	1116	WP_065904746.1	serine hydrolase domain-containing protein	-
LH783_RS15790 (3263983)	3263983..3264327	+	345	WP_004205915.1	hypothetical protein	-
LH783_RS15795 (3264356)	3264356..3264889	+	534	WP_053810394.1	hypothetical protein	-
LH783_RS15800 (3264892)	3264892..3265098	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
LH783_RS15805 (3265240)	3265240..3266532	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
LH783_RS15810 (3266549)	3266549..3267871	-	1323	WP_064187239.1	type II toxin-antitoxin system HipA family toxin	Toxin
LH783_RS15815 (3267871)	3267871..3268137	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LH783_RS15820 (3268420)	3268420..3268746	-	327	WP_053810395.1	hydroxyisourate hydrolase	-
LH783_RS15825 (3268743)	3268743..3269243	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LH783_RS15830 (3269221)	3269221..3270600	-	1380	WP_017900377.1	nucleobase:cation symporter-2 family protein	-
LH783_RS15835 (3270946)	3270946..3272100	+	1155	WP_043519552.1	FAD-dependent urate hydroxylase HpxO	-
LH783_RS15840 (3272081)	3272081..3273004	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49026.36 Da
• Isoelectric Point: 8.6091

>T220606 WP_064187239.1 NZ_CP085197:c3267871-3266549 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T220606 NZ_CP117200:343383-343691 [Pantoea sp. SS70]
ATGCAATATTTTATTTATCGCAATACCAATAGGAACAGCGAGTACCCTTATCTGGTAGACGTTCAAAGTGAGATTATCGG
CGAACTGGCGTCACGAATCGTTATCCCGCTCTACCCTCTAAAGCAGTTTAAAAAAAAGCAAGTTGAACGACTTAATCCGG
TAATCAAGGTTGAAGGCGAGGAATTTCTGGTCATGACTCACGAAATGGCCAGTGTGCGCGTGTCGCTTCTAGGCGATCAG
GTGATGGATGCACGTGTGTATCGCCAGCGGATTAAAGACTCGGTTGATTTTGTCTTTGATGGGTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT220606 WP_004205921.1 NZ_CP085197:c3268137-3267871 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT220606 NZ_CP117200:343141-343380 [Pantoea sp. SS70]
ATGCCTTCTACAGCCGTAAGACAAAAAAAAACTGTCAGTGTGACACTTGAACCTGCATTACTCGAGCAGGCTAGAGAAGC
AGGGCTCAATTTATCCGCCATCCTATCTAAAGCTTTGCAACATGAAATTCGCACAACCCAAGCGGAAAGATGGAAGCGTG
AAAACAGTGAAGGTTTGCAGGAACTCAATCGCATAACCGAAGAACACGGTTTATTGTCGGATGAATACAGGACGTTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9