Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1463819..1464476 Replicon chromosome
Accession NZ_CP085197
Organism Klebsiella quasipneumoniae strain NDM-101

Toxin (Protein)


Gene name cptA Uniprot ID A0A2A5MNX1
Locus tag LH783_RS07020 Protein ID WP_004205323.1
Coordinates 1464066..1464476 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag LH783_RS07015 Protein ID WP_002916312.1
Coordinates 1463819..1464085 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH783_RS06990 (1458970) 1458970..1460403 - 1434 WP_004205314.1 6-phospho-beta-glucosidase BglA -
LH783_RS06995 (1460522) 1460522..1461250 - 729 WP_004205316.1 MurR/RpiR family transcriptional regulator -
LH783_RS07000 (1461300) 1461300..1461611 + 312 WP_004205318.1 N(4)-acetylcytidine aminohydrolase -
LH783_RS07005 (1461774) 1461774..1462433 + 660 WP_004205319.1 hemolysin III family protein -
LH783_RS07010 (1462590) 1462590..1463573 - 984 WP_049116109.1 tRNA-modifying protein YgfZ -
LH783_RS07015 (1463819) 1463819..1464085 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
LH783_RS07020 (1464066) 1464066..1464476 + 411 WP_004205323.1 protein YgfX Toxin
LH783_RS07025 (1464483) 1464483..1465004 - 522 WP_004205324.1 flavodoxin FldB -
LH783_RS07030 (1465105) 1465105..1466001 + 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
LH783_RS07035 (1466024) 1466024..1466737 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LH783_RS07040 (1466743) 1466743..1468476 + 1734 WP_004205327.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16035.83 Da        Isoelectric Point: 11.4778

>T220597 WP_004205323.1 NZ_CP085197:1464066-1464476 [Klebsiella quasipneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
DWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T220597 NZ_CP117199:c1087550-1087332 [Pantoea sp. SS70]
ATGAGCAACCAAGCCTTAACTAAAACAGATTATTTGATGCGACTGCGCCGCTGTCGCTCAATCGATACCCTCGAACGGGT
TATCGAAAAAAACAAGTATGAATTACCTGATAATGAACTCGCGGTATTCTATTCGGCAGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAGTACCGGTTTCTGTCTGGAAGTTCGTCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT220597 WP_002916312.1 NZ_CP085197:1463819-1464085 [Klebsiella quasipneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220597 NZ_CP117199:c1087952-1087575 [Pantoea sp. SS70]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTGTGTGAGAACCTGTTCGACGAAAGTATGGC
GACATTGACCGACAGCCATCATGGTTGGGTTAATGATCCTACTTCGGAAAGCAATTTACAGCTCAACGATTTGATTGAGC
ATATCGCTTCTTTCACGATGAATTACAAAATTAAGCACGTTGAAGATGAAGCCCTGATTACGCAAATCGATGAATATCTT
GACGATACCTTTATGCTGTTCAGCAGCTATGGAATCAATACTCAGGATCTACAACGGTGGCAGCGTTCAGCAAAACGCTT
ATTTAATCTGTTTGCCGAAGAGTGCGCTTATCTCCAACAACCTAGCCATTCCTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MNX1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References