Detailed information of TA system TA220595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 7261520..7262091
• Replicon: chromosome
• Accession: NZ_CP085193
• Organism: Streptomyces rapamycinicus NRRL 5491

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LIV37_RS30520
• Protein ID: WP_020870940.1
• Coordinates: 7261717..7262091 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A6G4AJ86
• Locus tag: LIV37_RS30515
• Protein ID: WP_020870939.1
• Coordinates: 7261520..7261717 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIV37_RS30495 (LIV37_30630)	7257160..7258065	+	906	WP_020870935.1	LysR family transcriptional regulator	-
LIV37_RS30500 (LIV37_30635)	7258149..7258802	+	654	WP_020870936.1	cysteine dioxygenase family protein	-
LIV37_RS30505 (LIV37_30640)	7258799..7259854	+	1056	WP_020870937.1	putative sulfate exporter family transporter	-
LIV37_RS30510 (LIV37_30645)	7259943..7261487	+	1545	WP_020870938.1	MFS transporter	-
LIV37_RS30515 (LIV37_30650)	7261520..7261717	+	198	WP_020870939.1	Arc family DNA-binding protein	Antitoxin
LIV37_RS30520 (LIV37_30655)	7261717..7262091	+	375	WP_020870940.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LIV37_RS30525 (LIV37_30660)	7262203..7263999	+	1797	WP_121824322.1	DEAD/DEAH box helicase	-
LIV37_RS30530 (LIV37_30665)	7264777..7265418	+	642	WP_044574552.1	helix-turn-helix domain-containing protein	-
LIV37_RS30535 (LIV37_30670)	7265634..7266419	+	786	WP_020870943.1	S16 family serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (12-119)

Antitoxin

• Arc-like DNA binding domain (4-30)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13886.71 Da
• Isoelectric Point: 4.5579

>T220595 WP_020870940.1 NZ_CP085193:7261717-7262091 [Streptomyces rapamycinicus NRRL 5491]
MHYLTLPELLNLAERLGADEVRDYGLLESALARPQASVFGQDAYPDVWQKAAALMESLARNHALVDGNKRLSWYATWVFL
HINGHPLNADFDVDEAERFVLSVCQGELDVPKIAEGLTRFTNGM

Nucleotide

• Length: 375 bp

>T220595 NZ_CP117199:132406-132699 [Pantoea sp. SS70]
ATGCAAACGGAACTGGAATTTGAGTGGGACCTTAGCAAAGCAGAGTCTAATCACCGCAAGCATGGCATCCGCTTCGAAAT
TGCCGTGCGCGTGTTTGATGATCCTTTTCATCTGTCTAGCCAGGATCGTTGTGAGAATGGCGAATATCGCTGGCAATCAA
TAGGTAATGTTATGGGCCATATTATTATTTTGGTGGCCCACACGGTACGTTTCGATCAGGGCACTGAGATTATTCGCATC
ATTAGTGCACGTCCTGCCACGCGCAATGAGAGGAGACGCTATGAGCAAGGTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7305.20 Da
• Isoelectric Point: 6.2170

>AT220595 WP_020870939.1 NZ_CP085193:7261520-7261717 [Streptomyces rapamycinicus NRRL 5491]
MAALNLRFTDEELDALRKRAEAEGRSMQSFAHDAVIAAINEHSRLFNEAAAHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT220595 NZ_CP117199:132686-132988 [Pantoea sp. SS70]
ATGAGCAAGGTTAGGTTTAAACGCAGCGAATTACCGCCGCTTTCGGATAGCCTGCAATCTGAACTGGACGCGCTAAGTCA
AAAGCCGGATAGCGAGATTGATTGCGGTGAAATCGCCCCGTTAAATGAACGTTTCTGGAACAGGGCTTCCAGCGGGCGAT
TTTATCGTCCGATTAAAACACAGGCTTCAGTCAGAATTGACGCGGATGTGCTTGAGTGGTTGAAAGCGCCGGGTAAAGGG
TACCAGACGCGTTTGAACGCCATTTTGCGCGCGGCAATGCTGCGCGAATTAGAAAGCAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A6G4AJ86