Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3575688..3576306 Replicon chromosome
Accession NZ_CP085188
Organism Escherichia coli strain EC195

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LIN52_RS17350 Protein ID WP_001291435.1
Coordinates 3576088..3576306 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LIN52_RS17345 Protein ID WP_000344800.1
Coordinates 3575688..3576062 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN52_RS17335 (3570777) 3570777..3571970 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LIN52_RS17340 (3571993) 3571993..3575142 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LIN52_RS17345 (3575688) 3575688..3576062 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LIN52_RS17350 (3576088) 3576088..3576306 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LIN52_RS17355 (3576478) 3576478..3577029 + 552 WP_000102568.1 maltose O-acetyltransferase -
LIN52_RS17360 (3577145) 3577145..3577615 + 471 WP_000136192.1 YlaC family protein -
LIN52_RS17365 (3577779) 3577779..3579329 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LIN52_RS17370 (3579371) 3579371..3579724 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LIN52_RS17380 (3580103) 3580103..3580414 + 312 WP_000409911.1 MGMT family protein -
LIN52_RS17385 (3580445) 3580445..3581017 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220585 WP_001291435.1 NZ_CP085188:3576088-3576306 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220585 NZ_CP117184:c4475606-4475238 [Salmonella enterica subsp. enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220585 WP_000344800.1 NZ_CP085188:3575688-3576062 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220585 NZ_CP117184:c4475824-4475603 [Salmonella enterica subsp. enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References