Detailed information of TA system TA220571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1344558..1345183
• Replicon: chromosome
• Accession: NZ_CP085188
• Organism: Escherichia coli strain EC195

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LIN52_RS06540
• Protein ID: WP_000911330.1
• Coordinates: 1344785..1345183 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LIN52_RS06535
• Protein ID: WP_000450524.1
• Coordinates: 1344558..1344785 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN52_RS06510 (1340361)	1340361..1340831	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LIN52_RS06515 (1340831)	1340831..1341403	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LIN52_RS06520 (1341549)	1341549..1342427	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LIN52_RS06525 (1342444)	1342444..1343478	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LIN52_RS06530 (1343691)	1343691..1344404	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LIN52_RS06535 (1344558)	1344558..1344785	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LIN52_RS06540 (1344785)	1344785..1345183	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LIN52_RS06545 (1345330)	1345330..1346193	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
LIN52_RS06550 (1346208)	1346208..1348223	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
LIN52_RS06555 (1348297)	1348297..1348638	+	342	Protein_1280	esterase	-
LIN52_RS06560 (1348720)	1348720..1349466	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T220571 WP_000911330.1 NZ_CP085188:1344785-1345183 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T220571 NZ_CP117181:4108318-4108602 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT220571 WP_000450524.1 NZ_CP085188:1344558-1344785 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT220571 NZ_CP117181:4108080-4108328 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33