Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4465092..4465589 Replicon chromosome
Accession NZ_CP085183
Organism Escherichia coli strain EC7518

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LIN51_RS21725 Protein ID WP_001173343.1
Coordinates 4465092..4465265 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LIN51_RS21730 Protein ID WP_000398619.1
Coordinates 4465293..4465589 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN51_RS21715 (4461762) 4461762..4463219 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LIN51_RS21720 (4463456) 4463456..4464973 + 1518 WP_001295074.1 lysine--tRNA ligase -
LIN51_RS21725 (4465092) 4465092..4465265 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LIN51_RS21730 (4465293) 4465293..4465589 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LIN51_RS21735 (4465816) 4465816..4466088 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
LIN51_RS21740 (4466100) 4466100..4466330 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LIN51_RS21745 (4466511) 4466511..4468142 + 1632 WP_001216487.1 sensor histidine kinase -
LIN51_RS21750 (4468139) 4468139..4468858 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T220553 WP_001173343.1 NZ_CP085183:c4465265-4465092 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T220553 NZ_CP117172:6144-6425 [Serratia proteamaculans]
ATGACCTATAAACTCAAGTTTGAAAAACGGGCTCTGAAAGAGTGGGAAAAGCTGGGACATCCGATCAGGGAGCAGTTGAA
AAAAAAGCTGGCTGAGCGATTAGAAAACCCTCATGTGCCAGCGTCCCGACTCAGTGGCCGCCAGAACCGCTATAAAATCA
AACTACGTTCATCTGGGTATCGACTGGTTTACGAAGTCATCGACAACGAAATCGTCCTGCTGGTGATCGCCGTTGGCAAA
CGGGCAGGGGATGAAGTCTATTTAACTGCCGACAAACGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT220553 WP_000398619.1 NZ_CP085183:c4465589-4465293 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT220553 NZ_CP117172:5903-6154 [Serratia proteamaculans]
ATGGCTTATCAGATTTTAACTACTACCGCAGCGTCGATAACCGACCTGAAAAAAAATCCGATGGGAACCGTGGCCGATGG
TGACGGTGAAGCGGTCGCGATCCTCAATCGCAACGAACCAGCGTTCTACTGCGTTCCCCCCGAGCTCTACGCCTATTATC
GTGAGCTGGCCGAAGATGCTGAGTTGAATCAAATCGCTGATGAGCGCATGAAAAATGCCGAGTTTGTGAGCGTTACCCTC
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References