Detailed information of TA system TA220549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3639627..3640464
• Replicon: chromosome
• Accession: NZ_CP085183
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LIN51_RS17720
• Protein ID: WP_000227784.1
• Coordinates: 3639922..3640464 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LIN51_RS17715
• Protein ID: WP_001297137.1
• Coordinates: 3639627..3639938 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS17690 (3634647)	3634647..3635594	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LIN51_RS17695 (3635616)	3635616..3637607	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LIN51_RS17700 (3637597)	3637597..3638211	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LIN51_RS17705 (3638211)	3638211..3638540	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LIN51_RS17710 (3638552)	3638552..3639442	+	891	WP_000971336.1	heme o synthase	-
LIN51_RS17715 (3639627)	3639627..3639938	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LIN51_RS17720 (3639922)	3639922..3640464	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LIN51_RS17725 (3640520)	3640520..3641455	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
LIN51_RS17730 (3641863)	3641863..3643227	+	1365	WP_001000974.1	MFS transporter	-
LIN51_RS17735 (3643355)	3643355..3643846	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LIN51_RS17740 (3644014)	3644014..3644925	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T220549 WP_000227784.1 NZ_CP085183:3639922-3640464 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T220549 NZ_CP117168:c4194106-4193720 [Serratia proteamaculans]
ATGTACATGTTTGATACCAATACGGTCAGCCAACTGTTCCGTCGACATCCCCGCTTATTGCGCGTAATGGAAAAGATCCC
GCCGTCTGCGGTCTGCATTTCCAGTATCACGGAGGCGGAATTGTTGTATGGCGTAGCCAAGCGGCAAAGTTTGGCGCTAA
AAGCGACCGTGGCGGCATTCCTGGATTCCGTGACGGTGTATGCCTGGGACCGGGAAGCCGCGCATTGCTACGGAACGATG
CGGGCGGATATGGCGAAAAAGGGGCGAGTAATGGGGGCTTTGGATCAGCTTATTGCCGCACACGCGCAGAGCCGTGGTGC
GACAATAGTGACTAACGATAAGGCGTTCGCCATGGTGCCGGGGCTGAGGGTCGAAGACTGGACCTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT220549 WP_001297137.1 NZ_CP085183:3639627-3639938 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT220549 NZ_CP117168:c4194372-4194109 [Serratia proteamaculans]
ATGGAAAGAGTCGCGAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGATTACCCGTGGAATTTGAATTTGATACAGA
TCGTGTGTATATCCGCCGCGATCGGGAGGGAAACGTGATTTTATCAAAGTATCCGGCAAAACCTGACAGTTGGGCTCCAC
TGCTGCAAATGATCGAGCAAACTCAGGTGCCGCAGGACTTCCTGGTGGCTGAGGATCGTCAACAGGGGATCGCCATCCGC
GATCCGTTCGGCGAGGAAGAGTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3