Detailed information of TA system TA220547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2622074..2622712
• Replicon: chromosome
• Accession: NZ_CP085183
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LIN51_RS12825
• Protein ID: WP_000813794.1
• Coordinates: 2622536..2622712 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LIN51_RS12820
• Protein ID: WP_001270286.1
• Coordinates: 2622074..2622490 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS12800 (2617227)	2617227..2618167	-	941	Protein_2502	ABC transporter permease	-
LIN51_RS12805 (2618168)	2618168..2619181	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LIN51_RS12810 (2619199)	2619199..2620344	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
LIN51_RS12815 (2620589)	2620589..2621995	-	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
LIN51_RS12820 (2622074)	2622074..2622490	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LIN51_RS12825 (2622536)	2622536..2622712	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LIN51_RS12830 (2622934)	2622934..2623164	+	231	WP_000494244.1	YncJ family protein	-
LIN51_RS12835 (2623256)	2623256..2625217	-	1962	WP_001593323.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LIN51_RS12840 (2625290)	2625290..2625826	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LIN51_RS12845 (2625918)	2625918..2627093	+	1176	WP_001236215.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2627133..2628281	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T220547 WP_000813794.1 NZ_CP085183:c2622712-2622536 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T220547 NZ_CP117168:c3486539-3486141 [Serratia proteamaculans]
ATGTTCAGCCACATGCTGGATACCAACATTGTGATTTACGTGATCAAGCGTCGGCCACTGGAAGTGCTGCAAAGATTTAA
TCAGCATGCCGGCAAAATGGTGATCTCGTCGGTCACTTATGCCGAGTTGATTCATGGAGTGGAAAAAAGTATGCGGCCTA
CAGAAAATGCCCGGGTGATTGACGACTTTGTCTCCCGTCTGGAGATACTGGATTACACGGCCAAAGCTGCCGCTCACTAC
GGTAATATTCGTGCCTGCCTGGAACGGCAGGGTACGCCAATCGGCATCAACGATCTGCATATTGCCGGACATGCTCGCAG
TGAAGGATTGGTGCTGGTGACCAATAATCTGCGTGAGTTTGAACGCGTTGACGGCCTGCGTCTGCAAAATTGGCTGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT220547 WP_001270286.1 NZ_CP085183:c2622490-2622074 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT220547 NZ_CP117168:c3486772-3486539 [Serratia proteamaculans]
ATGACATTAGGTTCTGTATTTACCAATAACCGCACCCAGGCCGTGCGTTTACCTGCTGAGGTCCGCTTTCCCGATTCCGT
TTCAAAAGTCTCGGTACGGGTGGTCGGTAAAGAGCGCATCCTTTCGCCGGTAGAAAATACCTGGGACAGTTTTTTCTGCA
CAGACAATCACGTCAGCGATGATTTTCTCAGCGAGAGGGCCGACCAGTATCAGTCTGAAAGGGAAGAATTTTGA

Structures

Toxin

• PDB: 6HPB