Detailed information of TA system TA220546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2505207..2506795
• Replicon: chromosome
• Accession: NZ_CP085183
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E0IX85
• Locus tag: LIN51_RS12330
• Protein ID: WP_001125454.1
• Coordinates: 2505473..2506795 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LIN51_RS12325
• Protein ID: WP_001296726.1
• Coordinates: 2505207..2505473 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS12325 (2505207)	2505207..2505473	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LIN51_RS12330 (2505473)	2505473..2506795	+	1323	WP_001125454.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LIN51_RS12335 (2507034)	2507034..2508557	+	1524	WP_000331940.1	site-specific integrase	-
LIN51_RS12340 (2508520)	2508520..2510190	+	1671	WP_000169430.1	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49307.90 Da
• Isoelectric Point: 8.8753

>T220546 WP_001125454.1 NZ_CP085183:2505473-2506795 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T220546 NZ_CP117168:2238010-2238106 [Serratia proteamaculans]
AACAAGCCTTGCACAAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT220546 WP_001296726.1 NZ_CP085183:2505207-2505473 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT220546 NZ_CP117168:c2238112-2237988 [Serratia proteamaculans]
ACAAACAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTTTGTGCAAGGCTTGTTCAGCCGTGCACTTTAAGAGTAG

Structures

Toxin

• AlphaFold DB: E0IX85