Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1084071..1084654 Replicon chromosome
Accession NZ_CP085183
Organism Escherichia coli strain EC7518

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag LIN51_RS05250 Protein ID WP_000254745.1
Coordinates 1084319..1084654 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LIN51_RS05245 Protein ID WP_000581937.1
Coordinates 1084071..1084319 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN51_RS05235 (1080410) 1080410..1081711 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
LIN51_RS05240 (1081759) 1081759..1083993 + 2235 WP_000226815.1 GTP pyrophosphokinase -
LIN51_RS05245 (1084071) 1084071..1084319 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LIN51_RS05250 (1084319) 1084319..1084654 + 336 WP_000254745.1 endoribonuclease MazF Toxin
LIN51_RS05255 (1084725) 1084725..1085516 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
LIN51_RS05260 (1085744) 1085744..1087381 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LIN51_RS05265 (1087469) 1087469..1088767 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-109)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T220535 WP_000254745.1 NZ_CP085183:1084319-1084654 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T220535 NZ_CP117166:3712926-3713312 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATCGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAAATATGACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT220535 WP_000581937.1 NZ_CP085183:1084071-1084319 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT220535 NZ_CP117166:3713305-3713625 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAAGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTTCATTTAATAGATAAAAAAATATTATTCATCTA
G

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3


Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References