Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 295003..295634 Replicon chromosome
Accession NZ_CP085183
Organism Escherichia coli strain EC7518

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag LIN51_RS01320 Protein ID WP_001259388.1
Coordinates 295003..295278 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LIN51_RS01325 Protein ID WP_000593555.1
Coordinates 295275..295634 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIN51_RS01305 (290007) 290007..291074 + 1068 WP_000361485.1 HlyD family secretion protein -
LIN51_RS01310 (291071) 291071..293806 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
LIN51_RS01315 (293806) 293806..294930 + 1125 WP_001314210.1 ABC transporter permease -
LIN51_RS01320 (295003) 295003..295278 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
LIN51_RS01325 (295275) 295275..295634 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LIN51_RS01330 (295754) 295754..296155 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LIN51_RS01335 (296161) 296161..296967 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LIN51_RS01340 (296964) 296964..297728 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LIN51_RS01345 (297728) 297728..298561 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LIN51_RS01350 (298558) 298558..299502 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T220531 WP_001259388.1 NZ_CP085183:295003-295278 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T220531 NZ_CP117166:c12815-12432 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCGCATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACCGACAATACGCTGGAAAACTTAACAGTGGAAGCGGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT220531 WP_000593555.1 NZ_CP085183:295275-295634 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT220531 NZ_CP117166:c13033-12812 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATCTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCAGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCGCTGACAAATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References